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I3261

Sigma-Aldrich

Indo 1-AM

93 - 100% purity

Synonym(s):

4-(6-Carboxy-2-indolyl)-4′-methyl-2,2′-(ethylenedioxy)dianiline-N,N,N′,N′-tetraacetic acid tetrakis(acetoxymethyl) ester

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About This Item

Empirical Formula (Hill Notation):
C47H51N3O22
CAS Number:
112926-02-0
Molecular Weight:
1009.91
MDL number:
MFCD00036955
UNSPSC Code:
12171500
PubChem Substance ID:
24896026
Pricing and availability is not currently available.

Product Name

Indo 1-AM, ~95% (HPLC)

Assay

~95% (HPLC)

Quality Level

100

fluorescence

λex 330 nm; λem 450 nm (with Calcium)
λex 356 nm; λem 478 nm in methanol

storage temp.

−20°C

SMILES string

CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)c1ccc(C)cc1OCCOc2cc(ccc2N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)-c3cc4ccc(cc4[nH]3)C(=O)OCOC(C)=O

InChI

1S/C47H51N3O22/c1-28-7-11-39(49(19-43(56)68-23-63-29(2)51)20-44(57)69-24-64-30(3)52)41(15-28)61-13-14-62-42-18-35(37-16-34-8-9-36(17-38(34)48-37)47(60)72-27-67-33(6)55)10-12-40(42)50(21-45(58)70-25-65-31(4)53)22-46(59)71-26-66-32(5)54/h7-12,15-18,48H,13-14,19-27H2,1-6H3

InChI key

CAWBRCOBJNWRLK-UHFFFAOYSA-N

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Application

Cell permeable fluorescent probe for Ca2+; lower affinity than FURA 2; gives a large shift in fluorescent emission from 482 nm to 398 nm upon Ca2+ binding.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
MKBW8742V
MKCF1669
MKCC4756
MKCC1633
MKBZ4230V

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CD2 sets quantitative thresholds in T cell activation.
Bachmann MF
The Journal of Experimental Medicine, 190, 1383-1383 (1999)
Methods in Cellular Imaging, Methods in Physiology (2013)
Simultaneous recording of calcium transients and reactive oxygen intermediates of human polymorphonuclear granulocytes in response to formyl-Met-Leu-Phe and the environmental agent sulfite.
Grundler W
Cytometry, 40, 219-219 (2000)
T Guenoun et al.
Anesthesiology, 92(2), 542-549 (2000-02-26)
Propofol is considered to be an anesthetic agent with few or no negative inotropic effects. This study evaluated a possible direct depressant effect of propofol on sarcoplasmic reticulum Ca2+ accumulation and cardiomyocytes. The effects of propofol on intracellular Ca2+ transients
E Roux et al.
American journal of respiratory cell and molecular biology, 19(3), 437-444 (1998-09-09)
We have previously observed that acrolein administered ex vivo to isolated airways alters the subsequent airway responsiveness. To examine the cellular mechanisms involved in this alteration, we have studied the effect of acrolein exposure on calcium signaling in myocytes freshly
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