the bottle for B8647 contains 465ml of concentrate. One option is to pour out the contents into a calibrated volumetric flask and add sufficient water to make it 1000 ml. Once reconstituted to 1 liter, the contents of the flask could then be returned to the bottle for B8647. However, this process may seem labor-intensive. Alternatively, most users will likely choose to add 535 ml of water to the 1 liter bottle of concentrate and mix well.
B8647
Brilliant Blue R Concentrate
methanol solution
Synonym(s):
Brilliant Blue R, Acid Blue 83, Brilliant indocyanin 6B, Coomassie Brilliant Blue R
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1 EA
NOK 1,030.00
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1 EA
NOK 1,030.00
About This Item
Empirical Formula (Hill Notation):
C45H44N3NaO7S2
CAS Number:
Molecular Weight:
825.97
Colour Index Number:
42660
Beilstein:
5718025
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
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Product Name
Brilliant Blue R Concentrate, suitable for SDS-PAGE, methanol solution
form
methanol solution
Quality Level
color
dark blue
suitability
suitable for SDS-PAGE
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=C/C(C=C5)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1
InChI
1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53,54);/q;+1/p-1
InChI key
NKLPQNGYXWVELD-UHFFFAOYSA-M
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Application
Brilliant Blue R concentrate is a methanol based stain especially designed for staining protein in polyacrylamide but will also stain agarose gels. The stain contains methanol and acetic acid so gels do not require fixing prior to staining.
Brilliant Blue R Concentrate has been used for protein band detection after nondenaturing gradient gel electrophoresis, isoelectric focussing and SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis).[1][2][3]
Brilliant Blue R Concentrate has been used for protein band detection after nondenaturing gradient gel electrophoresis, isoelectric focussing and SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis).[1][2][3]
For detection of protein bands following electrophoresis.
Reconstitution
After dilution with deionized water to a final volume of one liter, solution will contain 0.25% (w/v) Brilliant Blue R, 40% (v/v) methanol and 7% (v/v) acetic acid.
Legal Information
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Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 1
Target Organs
Eyes,Central nervous system
Storage Class Code
3 - Flammable liquids
WGK
WGK 3
Flash Point(F)
57.2 °F - closed cup
Flash Point(C)
14 °C - closed cup
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Articles
Explore the impact of water quality on protein electrophoresis and Western blotting. Learn about the role of ultrapure water and its effect on chemiluminescent signals in Western blotting.
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How should product B8647-1ea be used? Should the contents of the bottle be removed into a calibrated cylinder, water added to reach a final volume of 1 L, and then replaced all into the bottle?
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