GDS MPX Top 6 STEC is an automated nucleic acid amplification system for the detection of the “Top Six” non-O157 Shiga Toxigenic E. coli (STEC) in raw ground beef, raw beef trim, raw spinach, and raw mixed greens (baby lettuce and other young leafy green vegetables). The "Top Six" non-O157 STEC are defined as E. coli belonging to serogroups O103, O111, O121, O145, O26 or O45 that possess both the eae gene and at least one of the Shiga Toxin Genes stx1 or stx2. GDS MPX Top 6 STEC utilizes a proprietary IMS-based sample preparation procedure to isolate organisms belonging to 6 specific Top STEC O-serogroups (O103, O111, O121, O145, O26 and O45) prior to genetic analysis for the associated pathogenicity genes.
Features and Benefits
Following sample preparation with IMS against the Top 6 O groups, samples are analyzed with a single multiplex assay for the presence of (eae), stx1, and stx2
Single-step 10-18 h enrichment in mEHEC®, enrichment media common to all BioControl methods for E.coli O157:H7, Shiga Toxin genes, and Top STEC
Components
MPX Top STEC Amplification Tubes
Top 6 STEC Concentration Reagent
Resuspension Buffer Tq
Top STEC Wash Solution
Compatibility
BioControl GDS
Testing Method
PCR, Immunomagnetic Separation (IMS)
Legal Information
mEHEC is a registered trademark of Merck KGaA, Darmstadt, Germany
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