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SCT107

Sigma-Aldrich

BioTracker 555 Orange Cytoplasmic Membrane Dye

Live cell imaging lipophilic carbocyanine membrane dye suitable for long-term fluorescent cell labeling and cell tracking studies.

Synonym(s):

Lipophilic carbocyanine dye, Live cell imaging probe, Live cell membrane dye

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1 ML
NOK 3,000.00

NOK 3,000.00

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1 ML
NOK 3,000.00

About This Item

UNSPSC Code:
12352207
NACRES:
NA.47

NOK 3,000.00

Please contact Customer Service for Availability

technique(s)

cell based assay: suitable

detection method

fluorometric

shipped in

ambient

General description

Lipophilic carbocyanine dyes are widely used for labeling neurons in tissues by retrograde labeling, and to label membranes in a wide variety of cell types. The dyes are weakly fluorescent in aqueous phase, but become highly fluorescent in lipid bilayers. Staining is highly stable with low toxicity and very little dye transfer in between cells, making the dyes suitable for long-term cell labeling and tracking studies. When live cells are stained, the dyes label plasma membranes and also are taken up into endocytic compartments. Cells can be fixed either before or after staining, although permeabilization affects the staining pattern.

Unlike PKH dyes, BioTracker Cytoplasmic Membrane Dyes do not require a complicated hypoosmotic labeling protocol. They are ready-to-use dye delivery solutions that can be added directly to normal culture media to label suspended or adherent cells in culture.

Spectral Properties
Absorbance: 549nm
Emission: 565nm

Application

Live cell fluorescent imaging
Live cell imaging lipophilic carbocyanine membrane dye suitable for long-term fluorescent cell labeling and cell tracking studies.
Research Category
Cell Imaging
Research Sub Category
Live Cell Dye

Quality

Spectral Properties
Absorbance: 549nm
Emission: 565nm

Physical form

Liquid

Storage and Stability

Store BioTracker 555 Orange Cytoplasmic Membrane Dye at 2-8ºC. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Statements

H225,H319

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

55.4 °F - closed cup

Flash Point(C)

13 °C - closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Questions

1–2 of 2 Questions  

  1. If using this dye on paraformaldehyde-fixed cells alongside antibody-based immunofluorescent staining, when should membrane labeling be performed?

    1 answer

    1. It is recommended to fix and permeabilize the cells before staining with the BioTracker membrane dye and prior to performing immunofluorescent staining. To prevent dye redistribution, avoid using detergents after the staining step.

      Helpful?

  2. In order to stain fixed cells, do we have to use PBS or other buffer at room temperature instead of culture medium at 37oC like Biotracker 400 Blue Cytoplasmic Membrane dye?

    1 answer

    1. BioTracker 555 Orange Cytoplasmic Membrane Dye (Cat. no. SCT107) is a lipophilic carbocyanine dye. Lipophilic carbocyanine dyes like this product have also been used to stain cells or tissues after formaldehyde fixation.
      For a detailed protocol of the assay, please see the product data sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/876/849/sct107ds.pdf

      Helpful?

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