Pluripotent stem cells, including embryonic stem cells (ES cells) and induced pluripotent stem cells (iPSCs), have the capacity to give rise to differentiated progeny representative of all three germ layers (ectoderm, endoderm, and mesoderm). Characterization of human iPS cells includes alkaline phosphatase and antibody staining for Oct-4, Sox-2, Nanog, SSEA and TRA surface markers. These assays require cell fixation and are end-point assays.
The BioTracker 529 Green Pluripotent Stem Cell Dye is a live stem cell probe that selectively identifies undifferentiated human ES and iPS cells. The probe localizes at mitochondria in the human stem cells. The dye’s fluorescence is dependent on the activities of ABCB1 and ABCG2 on the cell membrane. The dye is suitable for both live cell imaging and flow cytometry applications of human pluripotent stem cells.
Store BioTracker 529 Green Pluripotent Stem Cell Dye at -20°C, desiccate and protect from light Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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