Anti-c-ErbB2/c-Neu (Ab-2), mouse monoclonal, clone 9G6, recognizes the ~185 kDa c-ErbB2/c-Neu SK-BR-3 cells and breast carcinoma. It is validated for IP and IHC on frozen and paraffin sections.
Recognizes the ~185 kDa c-ErbB2/c-Neu protein in SK-BR-3 cells and breast carcinoma tissue. Does not cross-react with v-ErbB2 or the EGF receptor.
This clone expresses the kappa light chain isotype. Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 Ag14 myeloma cells. Recognizes the ~185 kDa c-ErbB2/c-neu protein.
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Warning
Toxicity: Standard Handling (A)
Analysis Note
Negative Control Normal skin tissue or HepG2 cells
Other Notes
Immunoprecipitates the 185 kDa c-neu protein and has been reported to stain formalin-fixed paraffin-embedded tissue sections of human breast carcinomas overexpressing the c-neu protein (see application references). This antibody reacts with a cell surface epitope of c-neu but does not cross-react with the EGF receptor. In one study ductal carcinomas in situ displaying large-cell, comedo growth type, were stained while none of 16 ductal carcinomas in situ of small-cell, papillary, or cribiform growth type were stained by this antibody. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
nwg
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International journal of cancer, 131(12), 2808-2819 (2012-04-19)
A major obstacle in the successful treatment of cancer is the occurrence of chemoresistance. Cancer cells surviving chemotherapy and giving rise to a recurrence of the tumor are termed cancer stem cells and can be identified by elevated levels of
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