This product does not require activation. Note that this material has not been tested for use in cell culture. Suitability would have to be determined by the end user.
CC4000
Urokinase-type Plasminogen Activator
Synonym(s):
uPA
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100 μG
NOK 4,600.00
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100 μG
NOK 4,600.00
About This Item
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.41
Recommended Products
biological source
human
Quality Level
Assay
>98% (SDS-PAGE)
form
solid
manufacturer/tradename
Chemicon®
technique(s)
activity assay: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Gene Information
human ... PLAU(5328)
General description
A thrombolytic agent that converts plasminogen to plasmin. A mixture of high molecular weight and low molecular weight urokinase.
Product Source: Prepared from urine of healthy individuals
Physical form
Lyophilized from 200 mM manitol, 100 mM NaCl, 50 mM Tris-HCl, pH 7.5 and 0.1 % PEG.
Storage and Stability
Reconstitute using 200-400 μL dH2O. Store at -20°C. Following reconstitution, aliquot and freeze (-70°C). This product is stable for 2 year as supplied. Stock solutions are stable for up to 6 months at -70°C.
Analysis Note
High molecular weight urokinase (54 kD): 40%.Low molecular weight urokinase (33 kD): 50%.Transitory forms of urokinase: 10%
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Certificates of Analysis (COA)
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Customers Also Viewed
Thrombolytic therapy: a comparison between urokinase and streptokinase. From a national cooperative study.
W R Bell
Seminars in thrombosis and hemostasis, 2(1), 1-13 (1975-07-01)
Assay methods and standard preparations for plasmin, plasminogen and urokinase in purified systems, 1967-1968.
Johnson, A J, et al.
Thrombosis et Diathesis Haemorrhagica, 21, 259-272 (1969)
Sean T Zuckerman et al.
Biomaterials, 30(23-24), 3825-3833 (2009-05-16)
Mass spectrometry is a powerful proteomic tool enabling researchers to survey the global proteome of a cell. This technique has only recently been employed to investigate cell-material interactions. We had previously identified material scarcity and limited adherent cells as challenges
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Hello, I am interested in adding uPA to the medium of my cell culture of human pluripotent stem cell. Is product number CC4000 suitabl and ready to add to the medium, or does the protein needs activation before usage?
1 answer-
Helpful?
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Is CC4000, Urokinase-type Plasminogen Activator is in its active conformation and ready to be used in protease activity assays, or it needs to be activated before use?
Is CC4000, Urokinase-type Plasminogen Activator is in its active conformation and ready to be used in protease activity assays, or it needs to be activated before use?
1 answer-
This product is a combination of active (low molecular weight) and inactive (high molecular weight) urokinase. The composition of the CC4000 product is as follows:
Low molecular weight urokinase (33 kD) 50%
High molecular weight urokinase (54 kD) 40%
Transient forms of urokinase 10%
Activation prior to use is not required.
uPA is produced as an inactive form called pro-uPA which is 54KDa. Pro-uPA is cleaved by proteases to produce a low molecular weight active form that is 33 kDa. This cleavage by plasmin occurs in two steps. The transitory form is the result of the first cleavage step. The second cleavage of this transient form results in the active 33 kDa uPA. The link below may provide additional helpful information:Helpful?
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