341597

FGF-II, Mouse, Recombinant, E. coli

• Synonym(s): mFGF-II, Fibroblast Growth Factor-II
• UNSPSC Code: 12352202

Properties

• Assay: ≥95% (SDS-PAGE)
• Quality Level: 100
• form: lyophilized solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• impurities: ≤0.1 ng/μg Endotoxins (ng/μg FGF-2)
• color: white
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Full-length, recombinant, mouse basic fibroblast growth factor expressed in E. coli. A multi-functional factor with chemotactic activity for numerous cell types. It plays a role in the proliferation of neuronal precursors and in neuronal differentiation. A potent angiogenic factor both in vivo and in vitro. Activates phospholipase D in endothelial cells. Mouse FGFII shares 95% identity with human FGFII. Useful for studies of cell activation and signaling.

Biochem/physiol Actions

ED₅₀: ≤0.5 ng/ml as determined by the dose-dependent proliferation of BALB/3T3 cells.

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.

Reconstitute to 100 µg/ml with sterile H₂O.

Other Notes

Coulier, F. et al. 1997. J. Mol Evol.44, 43.
Claus, P. et al., 2003. J. Biol. Chem.278, 749.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10-13 - German Storage Class 10 to 13