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biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity (predicted by homology)
all
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Anti-Farnesyl, rabbit polyclonal, recognizes farnesyl in a wide variety of species. Also recognizes KLH. It is validated for use in ELISA and immunofluorescence.
Purified rabbit polyclonal antibody. Recognizes farnesyl.
Recognizes farnesyl. Also recognizes KLH.
Immunogen
farnesyl cysteine conjugated to KLH
Application
ELISA (1:1000)
Immunofluorescence (1:50)
Immunoblotting (not recommended; see comments)
Immunofluorescence (1:50)
Immunoblotting (not recommended; see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In PBS.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Baron, R., et al. 2000. Proc. Natl. Acad. Sci. USA97, 11626.
Lin, H.P., et al. 1999. J. Gen. Virol.80, 91.
Aspbury, R.A., et al. 1998. Biochim Biophys Acta1392, 265.
Gromov, P.S., et al. 1996. Electrophoresis17, 1728.
Zhang, F.L. & Casey, P.J. 1996. Annu. Rev. Biochem.65, 241.
Lin, H.P., et al. 1999. J. Gen. Virol.80, 91.
Aspbury, R.A., et al. 1998. Biochim Biophys Acta1392, 265.
Gromov, P.S., et al. 1996. Electrophoresis17, 1728.
Zhang, F.L. & Casey, P.J. 1996. Annu. Rev. Biochem.65, 241.
Recognizes proteins that have been isoprenylated by farnesyltransferase of geranylgeranyltransferase at the C-A-A-X motif. Also recognizes KLH. Not suitable for immunoblotting due to the hydrophobicity of the isoprenylated motif. Variables associated with assay conditions will dictate the optimal working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Massimo Bonora et al.
Cell stem cell, 31(3), 359-377 (2024-03-09)
Mitochondrial fatty acid oxidation (FAO) is essential for hematopoietic stem cell (HSC) self-renewal; however, the mechanism by which mitochondrial metabolism controls HSC fate remains unknown. Here, we show that within the hematopoietic lineage, HSCs have the largest mitochondrial NADPH pools
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