23-021-M

UBE1 Protein, active, 10 µg

Active, full length, recombinant human UBE1. N-terminal 6His-tagged. For use in Enzyme Assays. Functions as an E1 enzyme in ubiquitination assays.

• Synonym(s): Ubiquitin activating enzyme E1, Ubiquitin-like modifier-activating enzyme 1, UBA1, A1S9 Protein, UBE1
• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.41

Properties

• biological source: human
• Quality Level: 100
• recombinant: expressed in baculovirus infected Sf21 cells
• form: liquid
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable
• solubility: water: soluble
• NCBI accession no.: NM_003334

Description

General description

Research area: Cell Signaling

Application

Ubiquitination Cascade Comoponent: E1

Biochem/physiol Actions

UBE1 has a crucial function in initiating the ubiquitin-proteasome pathway by activating ubiquitin. Research has shown that purified UBE1 can activate and link UB to ubiquitin-conjugating enzyme E2s. The substantial quantity of purified UBE1 is valuable for in vitro research on the ubiquitin pathway and its structural studies.

Ubiquitin activating enzyme E1 (UBE1) catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. It is responsible for the primary ubiquitin-protein isopeptide bond formation and is a critical component for the initiation of in vitro conjugation reactions. UBE1 activates ubiquitin by the ATP dependent adenylation of its C-terminal glycine carboxyl group. This ubiquitin residue is then linked to the sulphydryl side chain moiety of a cysteine residue within UBE1 by forming a high-energy thiol ester bond, liberating free AMP. There are two active sites within the UBE1 molecule allowing it to accommodate two ubiquitin molecules at one time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. The activated ubiquitin is then transferred to the lysine of target proteins via the E2/E3 conjugation cascade.

Physical form

Purified using immobilized metal affinity chromatography.

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.