1.15946

Nile blue (hydrogen sulfate) (C.I. 51180)

for microscopy Certistain^®

• Synonym(s): Nile blue (hydrogen sulfate) (C.I. 51180), Nile blue
• Empirical Formula (Hill Notation): C₂₀H₂₁N₃O₅S
• CAS Number: 16650-83-2
• Molecular Weight: 415.46
• UNSPSC Code: 41116130

Properties

• Quality Level: 400
• form: solid
• composition: Dye, ≥90% spectrophotometric assay
• pH: 2.2 (20 °C, 10 g/L in H₂O)
• mp: >300 °C
• solubility: 50 g/L
• bulk density: 300 kg/m³
• εmax: 1630-1820 at 635-639 nm in 50% ethanol at 0.003 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-30°C
• InChI: 1S/C20H20N3O.H2O4S/c1-3-23(4-2)13-9-10-17-18(11-13)24-19-12-16(21)14-7-5-6-8-15(14)20(19)22-17;1-5(2,3)4/h5-12H,3-4,21H2,1-2H3;(H2,1,2,3,4)/q+1;/p-1
• InChI key: MMYKGGIXVBGGSZ-UHFFFAOYSA-M

Description

General description

Nile blue (hydrogen sulfate) (C.I. 51180) is a CERTISTAIN dry dye that is used for the preparation of a staining solution e.g. staining melanins and lipofuscins in histological sections of human origin.
As CERTISTAIN dye it is chemically quality-certified according to highest standards and strict specifications. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations.

Analysis Note

Dye content (spectrophotometrically): ≥ 90 %
Identity
- Hydrogen sulfate (as sulfur):
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (ethanol 50 %): 635 - 639 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.003 g/l; ethanol 50 %): 1630 - 1820
TLC-Test: passes test
Loss on drying (110 °C): ≤ 8 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany