Skip to Content
Merck
All Photos(3)

Documents

06-719

Sigma-Aldrich

Anti-LexA Antibody, DNA-binding region

Upstate®, from rabbit

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

E. coli

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

Escherichia coli K12 ... Lexa(948544)

General description

The anti-LexA Antibody allows detection of LexA protein and recombinant proteins fused to the LexA protein. This antibody can be used to detect expression of potential bait proteins constructed for use with the yeast two-hybrid system or other interaction trap systems. LexA adds about 22 to 25 kDa to your protein, depending on the number of extra amino acids.

Specificity

Recognizes LexA portion of bait fusion proteins used in the yeast two-hybrid system.

Immunogen

His-tagged DNA binding domain of LexA protein corresponding to amino acid residues 1-202

Application

Detect LexA with Anti-LexA Antibody, DNA-binding region (Rabbit Polyclonal Antibody), that has been shown to work in IP & WB.

Quality

routinely evaluated by immunoblot on LexA-pRB transfected yeast lysate

Target description

24 kDa

Physical form

Format: Purified

Analysis Note

Control
LexA-pRB transfected yeast cell extract

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Insertional chromatin immunoprecipitation: a method for isolating specific genomic regions.
Hoshino, A; Fujii, H
Journal of Bioscience and Bioengineering null
Direct identification of insulator components by insertional chromatin immunoprecipitation.
Fujita, T; Fujii, H
Testing null
Yu-Fan Chen et al.
Molecular and cellular biology, 36(15), 2039-2050 (2016-05-18)
Cohesin associates with distinct sites on chromosomes to mediate sister chromatid cohesion. Single cohesin complexes are thought to bind by encircling both sister chromatids in a topological embrace. Transcriptionally repressed chromosomal domains in the yeast Saccharomyces cerevisiae represent specialized sites
Seiji Tanaka
Genes to cells : devoted to molecular & cellular mechanisms, 24(12), 781-788 (2019-10-11)
Protein-protein interactions are one of the most basic and critical processes underlying biological functions. Thus, identification of the interacting proteins of a protein of interest and further elucidation of the roles of the interactions is critical for understanding the related
The SOS regulatory system of Escherichia coli.
Little, J W and Mount, D W
Cell, 29, 11-22 (1982)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service