Z704881
Condensed Protocols from Molecular Cloning: A Laboratory Manual
a single-volume adaptation of the three-volume third edition
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
ISBN-10:
0-87969771-7
ISBN-13:
978-0-87969771-6
UNSPSC Code:
55101509
publication info
J. Sambrook and D. Russell, Cold Spring Harbor Laboratory Press, 2006, 700 pp., soft cover
manufacturer/tradename
Cold Spring Harbor
Looking for similar products? Visit Product Comparison Guide
General description
Molecular Cloning: Condensed Protocols is a single-volume adaptation of the three-volume third edition of Molecular Cloning: A Laboratory Manual. The book contains only the step-by-step portions of the protocols, accompanied by the appendices. Each protocol is cross-referenced to the appropriate pages in the original book.
This affordable volume, designed for bench use, offers individual investigators the opportunity to have their own personal collection of short protocols from the world′s best-selling manual of molecular biology techniques.
This affordable volume, designed for bench use, offers individual investigators the opportunity to have their own personal collection of short protocols from the world′s best-selling manual of molecular biology techniques.
Table of Contents
1. Using Plasmid Vectors in Molecular Cloning
2. Bacteriophage λ and its Vectors
3. Working with Bacteriophage M13 Vectors
4. Working with High Capacity Vectors
5. Gel Electrophoresis of DNA and Pulsed Field Agarose Gel Electrophoresis
6. Preparation and Analysis of Eukaryotic Genomic DNA
7. Extraction, Purification and Analysis of mRNA from Eukaryotic Cells
8. In Vitro Amplification of DNA by the Polymerase Chain Reaction
9. Preparation of Radiolabeled DNA and RNA Probes
10. Working with Synthetic Oligonucleotide Probes
11. Preparation of cDNA Libraries and Gene Identification
12. DNA Sequencing
13. Mutagenesis
14. Screening Expression Libraries
15. Expression of Cloned Genes in Escherichia coli
16. Introduction of Cloned Genes into Cultured Mammalian Cells
17. Analysis of Gene Expression in Mammalian Cells
18. Protein Interaction Technologies
Appendices
Index
2. Bacteriophage λ and its Vectors
3. Working with Bacteriophage M13 Vectors
4. Working with High Capacity Vectors
5. Gel Electrophoresis of DNA and Pulsed Field Agarose Gel Electrophoresis
6. Preparation and Analysis of Eukaryotic Genomic DNA
7. Extraction, Purification and Analysis of mRNA from Eukaryotic Cells
8. In Vitro Amplification of DNA by the Polymerase Chain Reaction
9. Preparation of Radiolabeled DNA and RNA Probes
10. Working with Synthetic Oligonucleotide Probes
11. Preparation of cDNA Libraries and Gene Identification
12. DNA Sequencing
13. Mutagenesis
14. Screening Expression Libraries
15. Expression of Cloned Genes in Escherichia coli
16. Introduction of Cloned Genes into Cultured Mammalian Cells
17. Analysis of Gene Expression in Mammalian Cells
18. Protein Interaction Technologies
Appendices
Index
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service