T7527

Tris-Borate-EDTA buffer

BioReagent, for molecular biology, 5x concentrate, DNase and RNase, none detected, powder blend, suitable for electrophoresis

• Synonym(s): TBE buffer
• MDL number: MFCD00236358
• UNSPSC Code: 41105319
• PubChem Substance ID: 329826869
• NACRES: NA.25

Properties

• grade: for molecular biology
• product line: BioReagent
• form: powder blend
• impurities: DNase and RNase, none detected
• pH: 8.1-8.5 (5 ×)
• solubility: water: 85.1 g/L, clear, colorless
• suitability: suitable for electrophoresis
• application(s): diagnostic assay manufacturing
• SMILES string: OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H
• InChI key: OSBLTNPMIGYQGY-UHFFFAOYSA-N

Description

Application

Ready for use in gel electrophoresis after dilution to working concentrations.

TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids.
Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Packaging

1L poly bottle contains a powder blend that can be dissolved and reconstituted within the bottle to prepare one liter of a 5× concentrate.
4L poly bottle contains a powder blend that can be dissolved and reconstituted to prepare four liters of a 5× concentrate.

Preparation Note

Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).

Reconstitution

Produces a 5× concentrate (0.445 M Tris-borate, 10 mM EDTA, pH 8.3) after dissolving with the indicated amount of water.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H360FD
• Precautionary Statements: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Repr. 1B
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves