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SML1661

Sigma-Aldrich

Bafilomycin A1

from Streptomyces griseus, ≥90% (HPLC), DMSO solution, V-ATPase inhibitor

Synonym(s):

BafA1

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About This Item

Empirical Formula (Hill Notation):
C35H58O9
CAS Number:
88899-55-2
Molecular Weight:
622.83
UNSPSC Code:
12352200
NACRES:
NA.77

product name

Bafilomycin A1 Ready Made Solution, 0.16 mM in DMSO, from Streptomyces griseus

biological source

Streptomyces griseus

Quality Level

200

form

DMSO solution

concentration

0.16 mM in DMSO

shipped in

dry ice

storage temp.

−20°C

InChI

1S/C35H58O9/c1-19(2)32-24(7)27(36)18-35(40,44-32)26(9)31(38)25(8)33-28(41-10)14-12-13-20(3)15-22(5)30(37)23(6)16-21(4)17-29(42-11)34(39)43-33/h12-14,16-17,19,22-28,30-33,36-38,40H,15,18H2,1-11H3/b14-12+,20-13+,21-16+,29-17-/t22-,23+,24-,25-,26-,27+,28-,30-,31+,32+,33+,35+/m0/s1

InChI key

XDHNQDDQEHDUTM-JQWOJBOSSA-N

General description

Bafilomycin A1, a macrolide antibiotic, belongs to the pleomacrolides family. It acts as a potent and selective inhibitor of vacuolar-type H+-ATPase. BafA1 can inhibit the viability of MG63 osteosarcoma cells. It can also stimulate mitochondrial dysfunction. BafA1 inhibits the proliferation of different types of cancer cells.

Application

Bafilomycin A1 has been used:
  • as an endosome acidification inhibitor to study the importance of endosome acidification in the extracellular vesicle uptake and cytosolic release of stably expressing NanoLuc luciferase-tagged Hsp70 (NLuc-Hsp70) in HeLa cells
  • as a vacuolar-type H+-ATPase (V-ATPase) inhibitor to study its effects on autophagic turnover of light chain 3 β (LC3-II) in mice
  • as an autophagy inhibitor to study its effects on primary rat liver sinusoidal endothelial cells (LSECs) defenestration

Biochem/physiol Actions

Bafilomycin A1 inhibits autophagy. It may exhibit anti-tumorigenic, anti-parasitic, and anti-neurodegenerative effects. Bafilomycin A1, found in lysosomes and endosomes prevents the acidification of these cell organelles. It also participates in blocking autophagosome-lysosome fusion and autolysosome acidification, steps necessary for maintaining the autophagic flux and cellular homeostasis.
Bafilomycin A1 is a macrolide antibiotic. Bafilomycin A1 acts as a potent and selective inhibitor of vacuolar-type H+-ATPase.

Other Notes

Bafilomycin A1 solution is provided in concentration of 0.16 mM. Typical concentrations for use in cell culture are 50-100 nM.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Bafilomycin A1 inhibits autophagy and induces apoptosis in MG63 osteosarcoma cells
Xie Z, et al.
Molecular medicine reports (2014)
Bafilomycin A1 induces caspase-independent cell death in hepatocellular carcinoma cells via targeting of autophagy and MAPK pathways
Yan Y, et al.
Scientific Reports (2016)
Molecular basis of V-ATPase inhibition by bafilomycin A1
Wang R, et al.
Nature Communications (2021)
Célia Fourrier et al.
Biochemical and biophysical research communications, 534, 107-113 (2020-12-15)
Measurement of autophagic flux in vivo is critical to understand how autophagy can be used to combat disease. Neurodegenerative diseases have a special relationship with autophagy, which makes measurement of autophagy in the brain a significant research priority. Currently, measurement of
Caroline Mauvezin et al.
Autophagy, 11(8), 1437-1438 (2015-07-15)
Autophagosome-lysosome fusion and autolysosome acidification constitute late steps in the autophagic process necessary to maintain functional autophagic flux and cellular homeostasis. Both of these steps are disrupted by the V-ATPase inhibitor bafilomycin A1, but the mechanisms potentially linking them are
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