SAB5700590

Recombinant 2019-nCoV Spike RBD Protein with His tag

• Synonym(s): 2019-nCoV RBD Protein, NCP-CoV RBD Protein, S glycoprotein Subunit1 RBD Protein, S1-RBD protein, novel coronavirus RBD Protein
• UNSPSC Code: 12352203
• NACRES: NA.26

Properties

• recombinant: expressed in HEK 293 cells
• Quality Level: 100
• Assay: >95% (HPLC); >95% (SDS-PAGE)
• form: lyophilized powder
• mol wt: 25.9 kDa
• impurities: <0.1 EU/μg protein (LAL test)
• UniProt accession no.: P0DTC2
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: SARS virus ... S(43740568)

Description

General description

Recombinant 2019-nCoV Spike RBD-His Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg319-Phe541) of 2019-ncov Spike RBD-His (Accession #YP_009724390.1) fused with a 6XHis tag at the C-terminus.

Biochem/physiol Actions

Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-COV-2 Spike RBD-His at 2μg/mL (100 μL/well) can bind Recombinant Human ACE2 with a linear range of 0.15-4.53 ng/mL. Immobilized human ACE2 on COOH Chip, can bind SARS-COV-2 Spike RBD Protein with an affinity constant of 36.7 nM as determined in a SPR assay.

Reconstitution

For lyophilized protein : Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

Storage and Stability

Store the lyophilized protein at -20°C to -80°C for long term. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8 °C for up to 1 week. This product is stable at ≤ -70°C for up to 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Data presented is the available current product information and provided as-is. These products have not been tested or verified in any additional applications, sample types, including any clinical use. Experimental conditions must be empirically derived by the user. Our Antibody Guarantee only covers tested applications stated herein and conditions presented in our product information and is not extended to publications.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable