SAB4700351

Monoclonal Anti-IgE antibody produced in mouse

clone BE5, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-IGHE
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: BE5, monoclonal
• form: buffered aqueous solution
• species reactivity: human
• concentration: 1 mg/mL
• technique(s): flow cytometry: suitable
• isotype: IgG1
• NCBI accession no.: NC_000014.8
• UniProt accession no.: P01854
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... IGHE(3497)

Description

General description

Immunoglobulin E (IgE) is encoded by the gene mapped to human chromosome 14q32.33. IgE belongs to the immunoglobulin family and consists of epsilon(ε) heavy chain in the constant (C) region. IgE has a monomeric structure with an extra domain in the constant region.

The antibody BE5 reacts with human IgE; it recognizes an epitope different from the ones recognized by 4G7 and 4H10 antibodies to IgE.

Immunogen

Purified human IgE

Application

The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 5 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Biochem/physiol Actions

Immunoglobulin E (IgE) binds very tightly to Fc receptors on basophils and mast cells. Thus, it plays a major role in stimulating allergic reactions. Elevated expression of the gene has been associated with the development of hyper IgE Syndrome (HIES), hay fever, atopic skin diseases such as eczema, anaphylactic shock and IgE-myeloma. In addition, overexpression of IgE is also observed in allergy/asthma. Therefore, increased levels of serum IgE can be considered as a diagnosis for asthma. IgE expression is down-regulated in congenital agammaglobulinemia and hypogammaglobulinemia because of faulty metabolism or synthesis of immunoglobulins.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Solution in Tris buffered saline, pH 8.0, with 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable