SAB4200451

Anti-NMD3 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-60S Ribosomal export protein NMD3, Anti-CGI-07, Anti-NMD3 homolog (S. cerevisiae)
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~58 kDa
• species reactivity: human, rat, mouse
• concentration: ~1.0 mg/mL
• technique(s): indirect immunofluorescence: 2.5-5 μg/mL using human HeLa cells.; western blot: 2.5-5.0 μg/mL using whole extracts of HEK-293T cells over-expressing mouse NMD3.
• UniProt accession no.: Q96D46
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... NMD3(51068) ; mouse ... Nmd3(97112) ; rat ... Nmd3(310512)

Description

General description

NMD3 ribosome export adaptor (NMD3) is an adaptor for 60S subunit export via the export receptor CRM1 (XPO1). In human, the C-terminal domain of NMD3 comprises a CRM1- dependent leucine-rich nuclear export signal (NES) and a nuclear localization signal (NLS) in its. Two zinc-binding centres and the ribosome-binding domain forms the N-terminal domain.

Immunogen

peptide corresponding to an internal region of human NMD3, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey, bovine and dog.

Application

Anti-NMD3 antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

NMD3 ribosome export adaptor (NMD3) is a highly conserved nucleocytoplasmic shuttling protein, essential for nuclear export of 60S ribosomal subunits.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable