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PLA0080

Sigma-Aldrich

Rabbit anti-PCNA Antibody, Affinity Purified

Powered by Bethyl Laboratories, Inc.

Synonym(s):

ATLD2, DNA polymerase delta auxiliary protein, PCNA, cyclin

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

grade

Powered by Bethyl Laboratories, Inc.

species reactivity

mouse, human

technique(s)

immunohistochemistry: 1:2,000-1:10,000
immunoprecipitation (IP): 2- 5 μg/mg
western blot: 1:5,000- 1:20,000

accession no.

P12004

UniProt accession no.

P12004

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

rabbit ... PCNA(5111)

Immunogen

The epitope recognized by PLA0080 maps to a region between residue 225 and the C-terminus (residue 261) of human proliferating cell nuclear antigen using the numbering given in SwissProt entry P12004 (GeneID 5111).

Physical form

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Other Notes

PCNA (Proliferating Cell Nuclear Antigen) was first identified as a factor expressed in the nuclei of replicating cells. PCNA plays a role in both DNA synthesis and repair. PCNA functions as a processivity factor for DNA polymerase by acting as a DNA clamp that tethers the polymerase catalytic units to the DNA template. The structure of PCNA is that of a trimeric ring that encircles and slides along replicating DNA. PCNA interacts with and is regulated by several proteins involved in cell cycle control and check point processes.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Kyuho Jeong et al.
Cardiovascular research, 118(4), 1150-1163 (2021-04-12)
Vascular smooth muscle cells (VSMCs) normally exhibit a very low proliferative rate. Vessel injury triggers VSMC proliferation, in part, through focal adhesion kinase (FAK) activation, which increases transcription of cyclin D1, a key activator for cell cycle-dependent kinases (CDKs). At
James M Murphy et al.
The Journal of biological chemistry, 298(6), 102013-102013 (2022-05-08)
Dysregulation of cyclin-dependent kinases (CDKs) can promote unchecked cell proliferation and cancer progression. Although focal adhesion kinase (FAK) contributes to regulating cell cycle progression, the exact molecular mechanism remains unclear. Here, we found that FAK plays a key role in

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