P350-05
Porcine Coronary Artery Smooth Muscle Cells: PCASMC (Cryovial)
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About This Item
UNSPSC Code:
41106514
NACRES:
NA.81
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biological source
Porcine coronary artery
Quality Level
form
solid
packaging
pkg of 500,000 cells
manufacturer/tradename
Cell Applications, Inc
growth mode
Adherent
karyotype
2n = 38
morphology
smooth muscle
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
diabetes; stroke; cardiovascular diseases
shipped in
dry ice
storage temp.
−196°C
Related Categories
General description
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PCASMC were used to investigate the role of c-Jun in smooth muscle proliferation, and to show that c-Jun inhibition abrogates smooth muscle cell growth and intimal thickening after arterial injury (Khachigian, 2002). PCASMC were also used to develop poly (l-lactide-co-caprolactone) nanofibrous scaffold material with potential for tissue engineering (Dong, 2008).
Characterization: positive for smooth muscle cell specific alpha-actin expression.
PCASMC were used to investigate the role of c-Jun in smooth muscle proliferation, and to show that c-Jun inhibition abrogates smooth muscle cell growth and intimal thickening after arterial injury (Khachigian, 2002). PCASMC were also used to develop poly (l-lactide-co-caprolactone) nanofibrous scaffold material with potential for tissue engineering (Dong, 2008).
Characterization: positive for smooth muscle cell specific alpha-actin expression.
Cell Line Origin
Artery
Application
vascular research, supply of blood to heart muscle
Components
Porcine Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO
Preparation Note
- 2nd passage, >500,000 cells in Porcine Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO
- Can be cultured at least 16 doublings
Subculture Routine
Please refer to the PCASMC Culture Protocol.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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In Young Choi et al.
Biomaterials, 269, 120222-120222 (2020-08-02)
Stem cell fate is largely determined by cellular signaling networks and is heavily dependent on the supplementation of exogenous recombinant proteins into culture media; however, uneven distribution and inconsistent stability of recombinant proteins are closely associated with the spontaneous differentiation
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