Skip to Content
Merck
All Photos(2)

Documents

MAK056

Sigma-Aldrich

Choline/Acetylcholine Quantification Kit

sufficient for 100 colorimetric or fluorometric tests (for choline assay and 50 tests acetylcholine assay)

Synonym(s):

Choline/Acetylcholine Assay Kit

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
12161503
NACRES:
NA.25

usage

sufficient for 100 colorimetric or fluorometric tests (for choline assay and 50 tests acetylcholine assay)

detection method

colorimetric
fluorometric

storage temp.

−20°C

General description

Choline is an essential nutrient, commonly grouped with the B complex vitamins, that plays key roles in many biological processes. Choline is a precursor for the synthesis of acetylcholine, a critical neurotransmitter in both the peripheral and central nervous systems.

Application

Choline/Acetylcholine Quantification Kit has been used to quantify the amounts of choline and acetylcholine in samples.

Suitability

Suitable for use with cell and tissue culture supernatants as well as serum, plasma, urine, and other biological fluids.

Principle

In this assay, choline concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the choline present. Acetylcholine levels can be determined by adding acetylcholinesterase to the reaction, which hydrolyzes acetylcholine to choline and acetate.

replaced by

Product No.
Description
Pricing

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H319,H334,H335

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Melis Emanet et al.
Frontiers in bioengineering and biotechnology, 10, 953867-953867 (2022-08-23)
Reactive oxygen species (ROS) are a common hallmark of many degenerative diseases, developing in all those cases where a failure of physiological antioxidant mechanisms occurs (in particular, antioxidant enzymes and the glutathione system), or in case of exposure to an
Elin Rebecka Carlsson et al.
Lipids in health and disease, 18(1), 169-169 (2019-09-07)
Phosphatidylcholine (PC), the most abundant of the phospholipids, has several metabolic functions in organs such as the liver and the intestine, important structural- and signaling functions in biological membranes, and might have a role in the effects of Roux-en-Y gastric
Compromised epidermal barrier stimulates Harderian gland activity and hypertrophy in ACBP-/-mice
Bek S, et al.
Journal of Lipid Research, jlr-M060780 (2015)
Lara Duran-Trio et al.
Journal of inherited metabolic disease, 45(2), 278-291 (2021-12-23)
Creatine (Cr) is a nitrogenous organic acid and plays roles such as fast phosphate energy buffer to replenish ATP, osmolyte, antioxidant, neuromodulator, and as a compound with anabolic and ergogenic properties in muscle. Cr is taken from the diet or
Effects of Hibernation on the Enteric Nervous System of the Thirteen-lined Ground Squirrels.
Eliades L, et al.
Journal of Undergraduate Research, 1-6 (2014)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service