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M221

Sigma-Aldrich

Monoclonal Anti-Nicotinic Acetylcholine Receptor (α3 Subunit) antibody produced in rat

~5 mg/mL, clone mAb 313, affinity isolated antibody, buffered aqueous solution

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About This Item

MDL number:
MFCD00677279
UNSPSC Code:
12352203

biological source

rat

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

mAb 313, monoclonal

form

buffered aqueous solution

species reactivity

chicken

concentration

~5 mg/mL

technique(s)

immunohistochemistry (frozen sections): 1:4,000

isotype

IgG2a

UniProt accession no.

P09481

shipped in

dry ice

storage temp.

−20°C

Gene Information

chicken ... CHRNA3(386578)

Specificity

Binds to native and denatured detergent-solubilized α3 subunit of AchR. Does not bind α1, α4, α5, α7, α8 and β2.

Immunogen

recombinant large cytoplasmic domain of the α3 subunit (amino acids 315-441) of the nicotinic acetylcholine receptor from chick.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Physical form

Solution in 20 mM Na3PO4, pH 7.2, containing 150 mM NaCl and 0.05% sodium azide.

Legal Information

Sold with permission of the Salk Institute

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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P J Whiting et al.
Brain research. Molecular brain research, 10(1), 61-70 (1991-04-01)
Neuronal nicotinic acetylcholine receptors (AChRs) are composed of two types of subunits: ACh-binding (termed alpha 2, alpha 3, alpha 4 ...) and structural (termed beta 2, beta 3, beta 4 ...). AChR subtypes composed of combinations of subunits of these
W G Conroy et al.
Neuron, 9(4), 679-691 (1992-10-01)
The acetylcholine receptor (AChR) alpha 5 gene has been classified as a member of the AChR gene family based on sequence homology. Expression studies, however, have yet to identify a function for the alpha 5 gene product or even to
Benjaporn Buranrat et al.
Oncology reports, 34(5), 2790-2796 (2015-09-10)
Ferritin is a major iron storage protein and essential for iron homeostasis. It has a wide range of functions in the body including iron delivery, immunosuppression, angiogenesis, and cell proliferation. Ferritin is overexpressed in many cancer cells, but its precise
Margarita Villar et al.
Biomolecules, 10(4) (2020-04-09)
Ticks are obligate hematophagous arthropods and vectors of pathogens affecting human and animal health worldwide. Cement is a complex protein polymerization substance secreted by ticks with antimicrobial properties and a possible role in host attachment, sealing the feeding lesion, facilitating
Lindstrom
Neurotransmissions, 12, 2-2 (1996)
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