M0195

MAPKAPK2 human

recombinant, expressed in HEK 293 cells, ≥60 units/mg protein, buffered aqueous glycerol solution

• Synonym(s): MAP kinase activated protein kinase 2, MAPKAP Kinase 2 human
• MDL number: MFCD04974881
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• recombinant: expressed in HEK 293 cells
• form: buffered aqueous glycerol solution
• specific activity: ≥60 units/mg protein
• mol wt: 45-60 kDa
• UniProt accession no.: P49137
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... MAPKAPK2(9261)

Description

Biochem/physiol Actions

MAPKAPK2 is a serine/threonine protein kinase activated by the p38 MAP kinase in response to various signals including mitogenic and stress stimuli. MAPKAPK2 is distinguished from the RSK family of ribosomal protein S6 kinases (also termed MAPKAPK1), which are activated by the MAP kinases, ERK1, and ERK2 based on its substrate specificity and amino acid sequence. Upon activation, p38 MAP kinase activates the downstream MAPKAPK2 by phosphorylation of the threonine residue in the Pro-Thr motif in response to heat shock or mitogenic stimuli. Activated MAPKAPK2 phosphorylates the small heat shock protein HSP25/HSP27, both in vitro and in vivo, at serine residues. Thus, HSP25/HSP27 seems to be the physiological substrate of MAPKAPK2.

Unit Definition

One unit will incorporate 1.0 nmole of phosphate into HSP 25 per minute at 30 °C at pH 7.4.

Physical form

Solution in 50 mM Tris-HCl, pH 7.4, 100 mM NaCl, 0.05 mM EDTA, 2 mM DTT, and 10% glycerol.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable