H5666
Granulocyte-Macrophage Colony-Stimulating Factor human
GM-CSF, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture
Synonym(s):
GM-CSF human
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
biological source
human
Quality Level
recombinant
expressed in HEK 293 cells
Assay
≥95% (SDS-PAGE)
form
lyophilized powder
potency
≤5 ng/mL EC50
quality
endotoxin tested
mol wt
dimer 15-36 kDa (glycosylated)
packaging
pkg of 5X10 μg
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/mg
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... CSF2(1437)
Looking for similar products? Visit Product Comparison Guide
Related Categories
General description
Granulocyte-Macrophage Colony-Stimulating Factor is mapped to human chromosome 5q21-q32.
Application
Granulocyte-Macrophage Colony-Stimulating Factor human has been used as a colony-stimulating cytokine in human acute myeloid leukemia cell line (OCI-M2) to test its effect on 5-azacitidin (AZA)-mediated DNA demethylation of upstream regulatory element (URE).
Biochem/physiol Actions
Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) is an immune modulator and proinflammatory cytokine. It binds to the heteromeric cell-surface receptors present in the immune cells like granulocytes, endothelial cells and macrophages. GM-CSF is implicated in inflammation associated disorder of the lung and renal system.
Granulocyte-macrophage colony stimulating factor (GM-CSF) is a growth and differentiation factor for cells in the granulocyte, macrophage and eosinophil lineage. GM-CSF stimulates colony formation from pluripotential progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. It also stimulates growth in some epithelial cells and osteoclasts. GM-CSF is produced by a variety of cell types (monocytes, endothelial cells, T-cells, fibroblasts, mitogen-stimulated B-cells, and LPS-stimulated macrophages). GM-CSF is secreted as a single chain glycoprotein containing 128 amino acids for human with a conserved disulfide bond. Human and murine GM-CSF share approx. 54% sequence homology and do not cross-react in bioactivity.
Caution
Although human and mouse GM-CSF share 54% amino acid sequence homology, their biological actions are species-specific and exhibit no cross-species reactivity.
Physical form
Lyophilized from a 0.2 μm filtered solution of 1x PBS.
Preparation Note
HumanKine GM-CSF is expressed as a 15-36 kDa glycosylated monomer in human HEK 293 cells. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications. Although human and mouse GM-CSF share 54% amino acid sequence homology, their biological actions are species-specific and exhibit no cross-species reactivity.
Analysis Note
The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).
Legal Information
HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
K Huebner et al.
Science (New York, N.Y.), 230(4731), 1282-1285 (1985-12-13)
Human granulocyte-macrophage colony-stimulating factor (GM-CSF) is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts directly on mature neutrophils. A full-length complementary DNA clone encoding human GM-CSF was used as a probe to screen a human
Yufang Shi et al.
Cell research, 16(2), 126-133 (2006-02-14)
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is an important hematopoietic growth factor and immune modulator. GM-CSF also has profound effects on the functional activities of various circulating leukocytes. It is produced by a variety of cell types including T cells, macrophages, endothelial
Stephanie S Watowich et al.
Immunological reviews, 238(1), 76-92 (2010-10-26)
Understanding the diversification of dendritic cell (DC) lineages is one of the last frontiers in mapping the developmental hierarchy of the hematopoietic system. DCs are a vital link between the innate and adaptive immune responses; thus, elucidating their developmental pathways
N Curik et al.
Leukemia, 26(8), 1804-1811 (2012-02-22)
Epigenetic 5-azacitidine (AZA) therapy of high-risk myelodysplastic syndromes (MDS) and acute myelogenous leukemia (AML) represents a promising, albeit not fully understood, approach. Hematopoietic transcription factor PU.1 is dynamically regulated by upstream regulatory element (URE), whose deletion causes downregulation of PU.1
Julie M Vose et al.
Leukemia & lymphoma, 50(9), 1412-1421 (2009-07-16)
In autologous stem cell transplantation, mobilized peripheral blood has replaced the bone marrow as the preferred source of hematopoietic stem cells (HSCs). Because HSCs normally exist in the blood in very low numbers, the use of agents to "mobilize" HSCs
Articles
Read article on hematopoietic cytokines and hematopoiesis
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service