CMC0015

BL21(DE3) pLysE Chemically Competent Cells

Escherichia coli, rod shaped

• UNSPSC Code: 41106202
• NACRES: NA.85

Properties

• product name: BL21(DE3) pLysE Chemically Competent Cells, for protein expression
• biological source: Escherichia coli
• grade: for molecular biology
• growth mode: adherent or suspension
• morphology: rod shaped
• technique(s): microbiological culture: suitable
• cell transformation: competent cell type: chemically competent; transformation efficiency: ≥1 x 10⁷ cfu/μg
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

The BL21(DE3) pLysE Chemically Competent Cells produce T7 lysozyme to repress low-level transcription from the T7 promoter prior to IPTG induction, helping to stabilize inserts coding for deleterious products.
These cells have a higher level of repression by T7 lysozyme, offering the greatest control over expression of inserts.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Features and Benefits

• a transformation efficiency of >1 × 10⁷ cfu/μg.
• economical prices
• offering superior value for everyday protein expression work.

Components

• BL21(DE3) pLysE chemically competent cells
• pUC 19 transformation control DNA
• recovery medium for expression

Safety Information

• Storage Class Code: 10 - Combustible liquids