AFRC 17D1

94120825, Application: Fusion partner for hybridoma production

• UNSPSC Code: 41106514

Properties

• biological source: Horse x Mouse/Horse Hybridoma
• ImmunoDon: Horse peripheral lymphocytes
• Myeloma: NS0
• Reactivity: Application: Fusion partner for hybridoma production
• technique(s): cell culture | mammalian: suitable
• isotype: Not specified
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Horse peripheral blood lymphocytes x Mouse NS0

Cell Line Description

The heterohybridoma AFRC 17D1 was obtained by fusion of lymphocytes from ponies immunised with the influenza A equine 2 virus (isolateA/Equine/Newmarket/79 (H3N8)) with mouse myeloma NS0 cells. The hybridoma was made aminopterin-sensitive by selective growth in 8-azaguanine. This non-Ig-secreting cell line was used as fusion partner for the establishment of equine-mouse heterohybidomas.

Immunogen

Influenza A equine 2 virus

Application

Fusion partner for hybridoma production

Culture Medium

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 5% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C

Other Notes

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