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product name
PI.1 Ut, 90111909
biological source
raccoon uterus
growth mode
Adherent
karyotype
2n = 38
morphology
Fibroblast-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Racoon uterus
Cell Line Description
Derived from trypsinised uterine tissue of an adult female Racoon. Pl.1Ut was developed for studies of feline and canine viruses since the Racoon is one of the few animals which is susceptible to both.
Application
Virus studies: feline and canine viruses
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 2-4x10,000 cells/cm2 by washing 3 times with 0.255 trypsin or trypsin/EDTA; 5% CO2; 37°C. Finite lifespan; maximum 90 passages expected. Growth rate is very slow.
Other Notes
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Certificates of Analysis (COA)
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