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B5926

Sigma-Aldrich

10× REDTaq® PCR Reaction Buffer

To be used with REDTaq® DNA Polymerase

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About This Item

UNSPSC Code:
41106306
NACRES:
NA.52

Quality Level

200

form

liquid

color

colorless

storage temp.

−20°C

Related Categories

General description

10x REDTaq® PCR Reaction Buffer is a polymerase chain reaction buffer for use with REDTaq® DNA Polymerase (D4309).

Application

10× REDTaq® PCR Reaction Buffer has been used as a component of reaction mix:

  • for PCR detection of virulence genes from Campylobacter jejuni food and clinical isolates in BALB/c mice
  • for PCR detection of virulence genes of Campylobacter coli isolates from infected mice liver
  • for identifying K-ras mutated alleles by PCR-restriction fragment length polymorphism (RFLP) in patients with colorectal carcinoma

Legal Information

REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Christoph P Dieterle et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 10(2), 641-650 (2004-02-05)
The aim of this study was to identify K-ras mutations as marker for isolated tumor cells in liver, lymph node, and bone marrow specimens of colorectal cancer patients. To detect these, a PCR-RFLP assay was used with a sensitivity exceeding
Virulence comparison of human and poultry campylobacter Jejuni isolates in a mouse model
Vuvckovic D, et al.
Medical Research Engineering, 5(10) (2017)
Birte Meyer et al.
Microbiology (Reading, England), 153(Pt 7), 2026-2044 (2007-06-30)
Newly developed PCR assays were used to PCR-amplify and sequence fragments of the dissimilatory adenosine-5'-phosphosulfate (APS) reductase genes (aprBA) comprising nearly the entire gene locus (2.2-2.4 kb, equal to 92-94 % of the protein coding sequence) from 75 sulfate-reducing prokaryotes
Romain Marti et al.
Applied and environmental microbiology, 75(15), 4967-4974 (2009-06-16)
The objective of this study was to identify a microbial marker for pig manure contamination. We quantified the persistence of four dominant bacterial groups from the pig intestinal tract throughout manure handling at 10 livestock operations (including aerobic digestion) by
Erin M Symonds et al.
Applied and environmental microbiology, 75(5), 1402-1409 (2009-01-07)
Human fecal matter contains a large number of viruses, and current bacterial indicators used for monitoring water quality do not correlate with the presence of pathogenic viruses. Adenoviruses and enteroviruses have often been used to identify fecal pollution in the
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