Starting with total RNA or samples pre-enriched or microRNAs, the MystiCq® microRNA cDNA Synthesis Mix kit contains all the components necessary to convert mature microRNAs into cDNA templates for qPCR.
MIRRT
MystiCq® microRNA cDNA Synthesis Mix
Synonym(s):
microRNA cDNA Synthesis Mix
About This Item
Recommended Products
technique(s)
cDNA synthesis: suitable
shipped in
wet ice
storage temp.
−20°C
General description
Application
Features and Benefits
- Separate polyadenylation and reverse transcription steps allow for the possibility of optimization of protocol if necessary
- cDNA produced may be archived for future use
- Single RT reaction can provide up to 1000 qPCR reactions
- Includes a positive control primer for use with most human tissues
Packaging
Legal Information
Kit Components Only
- Poly (A) Tailing Buffer (5X)
- Human Positive Control Primer
- Nuclease-free Water
- MystiCq® Universal PCR Primer
- Poly (A) Polymerase
- microRNA cDNA Reaction Mix
- ReadyScript® Reverse Transcriptase
related product
Storage Class Code
10 - Combustible liquids
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Articles
Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR
Sigma’s Imprint RNA Immunoprecipitation Kit was used to copurify human argonaute 2 (Ago2)-associated RNAs from HeLa cells. MicroRNAs reverse transcribed and quantitating using Mysticq reagents.
PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.
Protocols
Method for purification, reverse transcription and quantitative PCR for MicroRNAs using Mysticq reagents
-
I want to work with the cDNA of mARN, can I synthetize the cDNA of the mARN starting from a Total ARN sample using this kit? Or does the kit only synthetize the cDNA from the microARN?
1 answer-
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service