95348
Abberior® STAR 470SX, NHS ester
for long Stokes STED and 2-color STED application
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352200
Recommended Products
Assay
≥80.0% (degree of coupling)
form
solid
solubility
DMF: 1 mg/mL, clear
fluorescence
λex 480 nm; λem 610-630 nm in PBS, pH 7.4
storage temp.
−20°C
General description
Abberior STAR 470SX is the latest development of long-Stokes-Shift dyes for STED microscopy. The dye can be excited from 450 to 480nm. It can substitute dyes like Chromeo™ 494. For STED, a depletion wavelength ~750 nm is recommended. It is therefore well suited for 2-color STED imaging as implemented in the Leica TCS STED Ti:Sa microscope.
Abberior STAR 470SX is the dye of choice for long Stokes STED applications in the orange fluorescent regime. The dye is particularly designed and tested for 2-color STED microscopy in combination with our STAR 635 using a single STED wavelength. The dye is our recommendation for usage in the Leica TCS STED Ti:Sa 2-color system.
Key Features
Absorption Maximum, λmax: 475 nm (MeOH),
477 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 30′400 M-1cm-1 (MeOH),
22′700 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.69
Correction Factor, CF280 = ε280/max: 0.47
Fluorescence Maximum, λfl: 609 nm (MeOH),
627 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 740 - 770 nm
Fluorescence Quantum Yield, η: 0.80 (EtOH)
Fluorescence Lifetime, τ: 3.9 (EtOH)
Abberior STAR 470SX is the dye of choice for long Stokes STED applications in the orange fluorescent regime. The dye is particularly designed and tested for 2-color STED microscopy in combination with our STAR 635 using a single STED wavelength. The dye is our recommendation for usage in the Leica TCS STED Ti:Sa 2-color system.
Key Features
- Designed for STED microscopy at ~750 nm
- Long Stokes′ shift (>130 nm) for 2-color applications
- Tested in the Leica TCS STED Ti:Sa 2-color system
Absorption Maximum, λmax: 475 nm (MeOH),
477 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 30′400 M-1cm-1 (MeOH),
22′700 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.69
Correction Factor, CF280 = ε280/max: 0.47
Fluorescence Maximum, λfl: 609 nm (MeOH),
627 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 740 - 770 nm
Fluorescence Quantum Yield, η: 0.80 (EtOH)
Fluorescence Lifetime, τ: 3.9 (EtOH)
Application
Abberior™ STAR 470SX goat anti-rabbit antibody has been used for STED (stimulated emission depletion) microscopy in Caco-2 cells.
Suitability
Designed and tested for fluorescent super-resolution microscopy
Other Notes
Legal Information
6538 is a trademark of American Type Culture Collection
Chromeo is a trademark of Active Motif Chromeon GmbH
abberior is a registered trademark of Abberior GmbH
related product
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Thommie Karlsson et al.
PLoS pathogens, 8(10), e1002953-e1002953 (2012-10-17)
Quorum sensing (QS) signaling allows bacteria to control gene expression once a critical population density is achieved. The Gram-negative human pathogen Pseudomonas aeruginosa uses N-acylhomoserine lactones (AHL) as QS signals, which coordinate the production of virulence factors and biofilms. These
Tim Grotjohann et al.
Nature, 478(7368), 204-208 (2011-09-13)
Lens-based optical microscopy failed to discern fluorescent features closer than 200 nm for decades, but the recent breaking of the diffraction resolution barrier by sequentially switching the fluorescence capability of adjacent features on and off is making nanoscale imaging routine. Reported
T A Klar et al.
Optics letters, 24(14), 954-956 (2007-12-13)
We overcame the resolution limit of scanning far-field fluorescence microscopy by disabling the fluorescence from the outer part of the focal spot. Whereas a near-UV pulse generates a diffraction-limited distribution of excited molecules, a spatially offset pulse quenches the excited
Stefan W Hell
Nature biotechnology, 21(11), 1347-1355 (2003-11-05)
For more than a century, the resolution of focusing light microscopy has been limited by diffraction to 180 nm in the focal plane and to 500 nm along the optic axis. Recently, microscopes have been reported that provide three- to
Volker Westphal et al.
Physical review letters, 94(14), 143903-143903 (2005-05-21)
Utilizing single fluorescent molecules as probes, we prove the ability of a far-field microscope to attain spatial resolution down to 16 nm in the focal plane, corresponding to about 1/50 of the employed wavelength. The optical bandwidth expansion by nearly
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service