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P1379
TWEEN® 20
TWEEN® 20
2 of 4 reviewers received a sample product or took part in a promotion
viscous liquid
Synonym(s):
Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate
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Select a Size
25 ML
€18.00
100 ML
€37.90
250 ML
€47.50
500 ML
€75.90
1 L
€90.60
1 KIT
€129.00
4 L
€220.00
6 X 1 L
€317.00
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25 ML
€18.00
100 ML
€37.90
250 ML
€47.50
500 ML
€75.90
1 L
€90.60
1 KIT
€129.00
4 L
€220.00
6 X 1 L
€317.00
About This Item
Recommended Products
description
non-ionic
Quality Level
Assay
>40% (GC)
form
viscous liquid
mol wt
~1228
composition
lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids)
concentration
≥40.0% (GC)
technique(s)
DNA extraction: suitable
RNA extraction: suitable
cell culture | mammalian: suitable
protein purification: suitable
impurities
≤3.0% water
refractive index
n20/D 1.468 (lit.)
CMC
0.06 mM (20-25°C)
acid number
≤2.2 mg/g
hydroxyl value
96‑108 mg/g
transition temp
cloud point 76 °C
density
1.095 g/mL at 25 °C (lit.)
HLB
16.7
suitability
complies for IR spectroscopy
SMILES string
CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO
InChI
1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3
InChI key
HMFKFHLTUCJZJO-UHFFFAOYSA-N
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General description
Tween® 20 is a nonionic surfactant and detergent widely applied in biochemical and life science research. Its predominant use involves the solubilization of membrane proteins during the isolation of membrane-protein complexes. Moreover, it serves as a component in various washing, blocking, dilution, and stabilization buffers. Additionally, it functions as a reagent for immunohistochemistry, encompassing techniques like Western blotting and ELISAs. In these applications, Tween 20 plays crucial roles such as preventing non-specific antibody binding, minimizing background staining, and ensuring the even distribution of reagents.
Surfactants, possessing both hydrophilic and hydrophobic components, fall into two main categories: ionic, which ionize in water (anionic, cationic, or ampholytic), and nonionic, which do not ionize. The discussed product is a nonionic surfactant with diverse applications such as emulsification, dispersion, solubilization, and detergent use. Additionally, it can serve as a solubilizer for membrane proteins.
Surfactants, possessing both hydrophilic and hydrophobic components, fall into two main categories: ionic, which ionize in water (anionic, cationic, or ampholytic), and nonionic, which do not ionize. The discussed product is a nonionic surfactant with diverse applications such as emulsification, dispersion, solubilization, and detergent use. Additionally, it can serve as a solubilizer for membrane proteins.
Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.[1]
Application
Non-ionic detergent
TWEEN 20 has been used as a component of:
TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).
TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.
TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.
Features and Benefits
- Highly versatile surfactant for your life science and biochemical research
- Excellent solubilising, dispersing and emulsifying agent
- Suitable for DNA & RNA extraction, mammalian cell culture, and protein purification
Packaging
P1379-1KT:
Each kit contains 3 x 100ML samples, each sample from a uniquely manufactured lot.
Each kit contains 3 x 100ML samples, each sample from a uniquely manufactured lot.
Preparation Note
TWEEN 20 is miscible in water (100mg/mL), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate. TWEEN 20 is insoluble in liquid paraffin and fixed oils.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Legal Information
TWEEN is a registered trademark of Croda International PLC
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
527.0 °F - Pensky-Martens closed cup
Flash Point(C)
275 °C - Pensky-Martens closed cup
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Articles
Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.
Protocols
This page shows how to solubilize membrane proteins with products from Cytiva.
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What is the Department of Transportation shipping information for this product?
1 answer-
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What is the percentage of Tween 20 in Product P1379?
1 answer-
P1379 is not diluted in any solvent. Thus it can be considered "100%" or the "neat" material.
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The appearance of Product P1379, Tween® 20, is clear dark yellow. Is this OK?
1 answer-
Yes. The specifications for P1379 are "clear yellow to yellow green viscous liquid".
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What is the source of Product P1379, Tween® 20? Is it from plant or animal source, or synthetic?
1 answer-
Origin information on any lot of Tween 20 is specific to that lot, and can be found on its Certificate of Origin (CofO), which may be accessed on the Sigma-Aldrich website under its specific product and lot number.
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Is Product P1379, Tween® 20, protease-free?
1 answer-
We have not tested P1379 in quality control to be protease-free. There is a Molecular Biology grade of Tween 20, P9416, but this is not tested specifically for proteases either. P9416 is tested to be endonuclease-endonuclease free and to be ribonuclease-free.
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