G6639

Guanidine thiocyanate

≥97% (titration)

• Synonym(s): Guanidinium rhodanide, Guanidinium thiocyanate
• Linear Formula: NH₂C(=NH)NH₂ · HSCN
• CAS Number: 593-84-0
• Molecular Weight: 118.16
• Beilstein: 3563461
• EC Number: 209-812-1
• MDL number: MFCD00013027
• UNSPSC Code: 12352209
• PubChem Substance ID: 24895264
• NACRES: NA.55

Properties

• biological source: synthetic
• Quality Level: 200
• Assay: ≥97% (titration)
• form: powder or crystals
• technique(s): RNA extraction: suitable
• color: white to off-white
• mp: 120-122 °C (lit.)
• solubility: water: 4 M, clear, colorless
• anion traces: chloride (Cl^-): ≤2.5%
• SMILES string: SC#N.NC(N)=N
• InChI: 1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H
• InChI key: ZJYYHGLJYGJLLN-UHFFFAOYSA-N

Description

Application

Guanidine thiocyanate has been used:

• as a denaturing agent to study its effects on surface activity of globular protein
• for the extraction of total RNA from primary hepatocytes
• as a component of the incubating buffer for extraction of DNA from human bone
• as a component of the guanidine thiocyanate (GITC) lysis buffer for extraction of elephant shark DNA
• as a component of lysis solution for extraction of RNA from rat pancreatic tissues

Chaotropic agent and strong denaturant; solubilizes cells.

Biochem/physiol Actions

Guanidine thiocyanate (GTC) is a powerful protein denaturant and can denature endogenous ribonucleases. It plays a role in separation of ribosomal ribonucleic acid (rRNA) from ribosomes. GTC in combination with phenol and chloroform can be used in isolation of total RNA from cells and tissues.

Safety Information

• Pictograms: GHS05,GHS07
• Signal Word: Danger
• Hazard Statements: H302 + H312 + H332,H314,H412
• Precautionary Statements: P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C
• Supplementary Hazards: EUH032
• Storage Class Code: 8B - Non-combustible corrosive hazardous materials
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves