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67515

Millipore

STEC Millichrome™ plus Supplement

suitable for microbiology

Synonym(s):

Shiga-Toxin producing E. coli (STEC) Chromogenic supplement

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About This Item

UNSPSC Code:
41171614
NACRES:
NA.74

sterility

aseptically filled

Quality Level

200

form

dry powder

shelf life

limited shelf life, expiry date on the label

technique(s)

microbiological culture: suitable

solubility

sterile water: 1 (vial/10ml)

application(s)

food and beverages
microbiology

shipped in

ambient

storage temp.

2-8°C

suitability

selective for bacteria: Escherichia coli (Shiga Toxin-STEC

General description

STEC Millichrome™ plus Supplement is a selective media supplement for the detection of E. coli (STEC). The distinguishing feature of these media is the inclusion of a chromogenic substrate mix that produces visible color changes upon enzymatic activity of E. coli (STEC).Millichrome™ chromogenic media is a specialized type of culture medium used to detect and isolate specific groups of bacteria based on their ability to produce enzymes with chromogenic substrates. The package contains 5 vials, each vial sufficient for 1 liter of final media. One vial of the supplement should be rehydrated with 10 mL of sterile water and then added to 1 liter of melted STEC Millichrome™ plus Agar base cooled at 45-50 °C.

Application

STEC Millichrome™ plus Supplement is a selective chromogenic media supplement intended for use in the qualitative detection, differentiation and presumptive identification of E. coli (STEC) from food, animal feed, and environmental samples.

Packaging

5 vials (1 vial is sufficient for 1 L of final media)

Preparation Note

  • Aseptically rehydrate 1 vial with 10 mL of sterile water.
  • Swirl well until complete dissolution.
  • Add 1 vial rehydrated solution to 1L STEC Millichrome® plus Agar base cooled at 45-50 °C.
  • Swirl gently to homogenize.

Analysis Note

The Shiga toxins E. coli is detected by a characteristic mauve color after only 24 h at an optimal growth temperature of 37°C, while most other Enterobacteriaceae form Colorless, blue, or inhibited after incubation at the same temperature

Legal Information

CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
MilliChrome is a trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H302

Precautionary Statements

P264 - P270 - P301 + P312 - P501

Hazard Classifications

Acute Tox. 4 Oral

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

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Certificates of Analysis (COA)

Lot/Batch Number
BCCJ8990

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Articles

Microbial Detection Using Chromogenic and Fluorogenic Culture Media

Selective media enable faster results and visual confirmation for the detection, identification, and enumeration of microorganisms

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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