GPT-RO
Roche
Glutamate-Pyruvate Transaminase (GPT)
from pig heart
Synonym(s):
alanine aminotransferase
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About This Item
Recommended Products
biological source
pig heart
Quality Level
form
suspension
specific activity
≥80 units/mg protein (At 25 °C with L-alanine and 2-oxoglutarate as the substrates.)
packaging
pkg of 1 mL (10105589001 [10 mg])
pkg of 2.5 mL (10737127001 [25 mg])
manufacturer/tradename
Roche
optimum pH
~8.0
shipped in
wet ice
storage temp.
2-8°C
Related Categories
General description
Glutamate-pyruvate transaminase is a blood enzyme. It metabolizes glutamate in blood resulting in low extracellular levels of brain glutamate.
Application
Synthesis of unnatural L-amino acids from α-keto acids.
Quality
Contaminants: < 0.01% GIDH, LDH, and MDH each, < 0.03% GOT
Unit Definition
Unit Conversion: 1 U [+25 °C, pH 7.5] ≈ 1.75 U [+37 °C, pH 7.5]
Physical form
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6
Preparation Note
Activator: Pyridoxalphosphate
Storage conditions (working solution): The enzyme is stable when dissolved in human serum and frozen in aliquots. For best results, centrifuge the enzyme and decant the supernatant before dissolving the enzyme in human serum to avoid high ammonium sulfate concentrations in the solution.
Storage conditions (working solution): The enzyme is stable when dissolved in human serum and frozen in aliquots. For best results, centrifuge the enzyme and decant the supernatant before dissolving the enzyme in human serum to avoid high ammonium sulfate concentrations in the solution.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
No data available
Flash Point(C)
No data available
Certificates of Analysis (COA)
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Francisco Campos et al.
Clinical science (London, England : 1979), 121(1), 11-17 (2011-01-27)
Ischaemic stroke is associated with an excessive release of glutamate in brain. GOT (glutamate-oxaloacetate transaminase) and GPT (glutamate-pyruvate transaminase) are two enzymes that are able to metabolize blood glutamate facilitating the lowering of extracellular levels of brain glutamate. Our aim
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