11699121001
Roche
PCR Buffer Set
suitable for PCR, pkg of 2 × 2 mL
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About This Item
UNSPSC Code:
41116133
Recommended Products
packaging
pkg of 2 × 2 mL
manufacturer/tradename
Roche
technique(s)
PCR: suitable
storage temp.
−20°C
General description
The kit-provided PCR buffer without MgCl2, in combination with the kit-provided MgCl2 stock solution, are used for the individual adjustment of the Mg2+ concentration. In most PCR applications, a concentration of 1.5 mM MgCl2 will yield satisfactory results with a dNTP concentration of 200 μM for each nucleotide. To optimize the PCR, titrate to determine the optimal Mg2+ concentration for high PCR specificity and amplicon yield.
Application
For optimization of the magnesium-chloride concentration in the polymerase chain reaction (PCR) using Taq DNA Polymerase.
Packaging
1 set containing 2 components
1 set containing 3 components
Quality
Function test: Each lot of buffer is tested in PCR.
Absence of contaminants: No exo- or endonuclease activity is detectable according to the current Quality Control procedures.
Absence of contaminants: No exo- or endonuclease activity is detectable according to the current Quality Control procedures.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Kit Components Only
Product No.
Description
- MgCl2 Stock Solution 25 mM
- PCR Buffer without Mg2+ 10x concentrated
- Alkaline Phosphatase Substrate, CSPD
- Enhancer, Emerald II
- Assay Buffer
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
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