Skip to Content
Merck
All Photos(1)

Documents

PC712

Sigma-Aldrich

Anti-p53 Binding Protein 1 (Ab-1) Rabbit pAb

liquid, Calbiochem®

Synonym(s):

Anti-53BP1

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

does not contain

preservative

species reactivity

mouse, human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−70°C

target post-translational modification

unmodified

Gene Information

human ... TP53(7157)

General description

Purified rabbit polyclonal antibody. Recognizes the ~230 kDa 53BP1 protein.
Recognizes the ~230 kDa 53BP1 protein in HeLa cell nuclear extract (Cat. No. WB64).
This Anti-p53 Binding Protein 1 (Ab-1) Rabbit pAb is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of p53 Binding Protein 1 (Ab-1).

Immunogen

Human
a recombinant protein consisting of human 53BP1 fused to a His•Tag sequence

Application

Immunoblotting (1:3000-1:5000)

Immunofluorescence (1:300-1:500, indirect)

Immunoprecipitation (1:300-1:500)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 100 mM NaCl, 50 mM Tris-HCl, 20% glycerol, pH 7.6.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Analysis Note

Positive Control
HeLa Cell Nuclear Extract (Cat. No. WB64)

Other Notes

Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Mariana Acevedo et al.
Cancer research, 76(11), 3252-3264 (2016-05-22)
Promyelocytic leukemia (PML) plays a tumor suppressive role by inducing cellular senescence in response to oncogenic stress. However, tumor cell lines fail to engage in complete senescence upon PML activation. In this study, we investigated the mechanisms underlying resistance to
Neylen Del Toro et al.
Cell cycle (Georgetown, Tex.), 18(6-7), 759-770 (2019-03-16)
Senescence is a tumor suppressor program characterized by a stable growth arrest while maintaining cell viability. Senescence-associated ribogenesis defects (SARD) have been shown to regulate senescence through the ability of the ribosomal protein S14 (RPS14 or uS11) to bind and
Shinji Kohsaka et al.
NPJ precision oncology, 8(1), 117-117 (2024-05-25)
E7820 and Indisulam (E7070) are sulfonamide molecular glues that modulate RNA splicing by degrading the splicing factor RBM39 via ternary complex formation with the E3 ligase adaptor DCAF15. To identify biomarkers of the antitumor efficacy of E7820, we treated patient-derived
Hiroshi Ikeuchi et al.
Oncogene (2024-08-03)
Identifying the mechanisms of action of anticancer drugs is an important step in the development of new drugs. In this study, we established a comprehensive screening platform consisting of 68 oncogenes (MANO panel), encompassing 243 genetic variants, to identify predictive
Mafuka Suzuki et al.
PloS one, 18(1), e0281168-e0281168 (2023-01-28)
Malignancy is often associated with therapeutic resistance and metastasis, usually arising after therapeutic treatment. These include radio- and chemo-therapies, which cause cancer cell death by inducing DNA double strand breaks (DSBs). However, it is still unclear how resistance to these

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service