MABN13

Anti-Amyloid β42 Antibody, clone G2-13

clone G2-13, from mouse

• Synonym(s): Alzheimer disease, Alzheimer disease amyloid protein, Cerebral vascular amyloid peptide, Protease nexin-II, amyloid beta (A4) precursor protein, amyloid beta A4 protein, amyloid beta precursor protein, beta-amyloid peptide, human mRNA for amyloid A4 prec
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• clone: G2-13, monoclonal
• species reactivity: human
• species reactivity (predicted by homology): mouse (based on 100% sequence homology)
• technique(s): ELISA: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_000475
• UniProt accession no.: P05067
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... APP(351)

Description

General description

The cerebral and vascular plaques associated with Alzheimer′s disease (AD) are mainly composed of amyloid beta peptides (Aβ). Aβ is derived from cleavage of the amyloid precursor protein (APP) and varies in length from 39 to 43 amino acids. Aβ [1-40], Aβ [1-42], and Aβ [1-43] peptides result from cleavage of APP after residues 40, 42, and 43, respectively. The cleavage takes place by gamma-secretase during the last APP processing step. Aβ [1-40], [1-42] and [1-43] peptides are major constituents of the plaques and tangles that occur in AD. These Amyloid beta antibodies and peptides have been developed as tools for elucidating the biology of AD.

Specificity

This antibody recognizes human Amyloid β42 isoform at the C-terminus

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of Amyloid β42.

Application

Anti-Amyloid β42 Antibody, clone G2-13 is an antibody against Amyloid β42 for use in WB, IH, ELISA.

Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected Amyloid β42 in Alzheimerdiseased cerebral cortex and hippocampus tissue.
ELISA: A representative lot of MABN12 antibody was used in a titer ELISA. Specificity of detection for Amyloid beta peptides is displayed below.


ELISA:

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

Quality

Evaluated by Western Blot on human Alzheimer diseased brain tissue lysate.

Western Blot Analysis: 1 µg/ml of this antibody detected Amyloid β42 in 10 µg of human Alzheimer diseased brain tissue lysate.

Target description

4kDa Calculated

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide and 1% BSA.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human Alzheimer diseased brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable