MABF2007

Anti-CHIKV E2 Antibody, clone 1.3A2

• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• antibody form: purified antibody
• clone: 1.3A2; monoclonal
• purified by: using protein G
• species reactivity: virus, mouse
• concentration: (Please refer to lot specific datasheet.)
• technique(s): ELISA: suitable; neutralization: suitable; western blot: suitable
• isotype: IgG2aκ
• UniProt accession no.: Q8JUX5

Description

General description

Sucrose gradient-purified, gamma-irradiated CHIKVMAU E2 protein (Mauritius strain 06113879) with Quil-A saponin as adjuvant followed by a second application and later a boost with binary-ethyleneimine inactivated CHIKVASIAN.

Specificity

Clone 1.3A2 specifically detects E2 glycoprotein in Chikungunya virus. May recognize multiple epitopes on E2 protein.

Application

Western Blotting Analysis: A 1:500 dilution from a representative lot detected E2 of Chikungunya virus in C6/36 mosquito cell lysates either mock-infected (negative) or CHIKV-infected (positive). (Courtesy of Jody Peters, Ph.D., University of Queensland, Australia).

Neutralizing Analysis: A representative lot efficiently neutralized different CHIKV isolates in Neutralizing applications (Goh, L.Y., et. al. (2013). Clin Immunol. 149(3):487-97).

Western Blotting Analysis: A representative lot detected CHIKV E2 in Western Blotting applications (Goh, L.Y., et. al. (2013). Clin Immunol. 149(3):487-97).

ELISA Analysis: A representative lot detected CHIKV E2 in ELISA applications (Goh, L.Y., et. al. (2013). Clin Immunol. 149(3):487-97).

Quality

Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed to be mouse IgG2a by isotype testing.

Physical properties

~50 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Physical form

Purified mouse monoclonal antibody IgG2a in PBS without azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Chikungunya virus.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.