MABE645

Anti-acetyl-Histone H3 (Lys9) Antibody, clone RM161

clone RM161, from rabbit

• Synonym(s): Histone H3.3, Histone H3
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: RM161, monoclonal
• species reactivity: human
• technique(s): multiplexing: suitable; western blot: suitable
• isotype: IgG
• UniProt accession no.: P84243
• shipped in: wet ice
• target post-translational modification: acetylation (Lys9)
• Gene Information: human ... H3F3B(3021)

Description

General description

Histone H3, also known as Histone H3.1t (H3/t), H3t, H3/g, and encoded by the gene name HIST3H3/ H3FT, is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Therefore, histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. High levels of phosphorylation of Histone H3 are associated with mitosis.

Specificity

This antibody reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with other acetylated Lysines in Histone H3.

Immunogen

A linear peptide corresponding to human Histone H3 acetylated at Lys9.

Application

This Anti-acetyl-Histone H3 (Lys9) Antibody, clone RM161 is validated for use in Western Blotting, Multiplexing for the detection of acetyl-Histone H3.

Quality

Evaluated by Western Blotting in untreated and sodium butyrate treated HeLa acid extract.

Western Blotting Analysis (WB): 0.25 μg/mL of this antibody detected acetyl-Histone H3 (Lys9) in sodium butyrate treated HeLa acid extract.

Target description

~17 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): does not flash
• Flash Point(C): does not flash