MAB4356
Anti-DPPA-2 Antibody, clone 6C1.2
clone 6C1.2, from mouse
Synonym(s):
Pluripotent embryonic stem cell-related gene 1 protein, developmental pluripotency associated 2
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
6C1.2, monoclonal
species reactivity
mouse
technique(s)
western blot: suitable
input
sample type: mouse embryonic stem cell(s)
isotype
IgG2aκ
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Dppa2(73703)
General description
DPPA-2 (developmental pluripotency associated-2) is a novel Oct-4-related protein that is localized to the nucleus of mouse fetal germ cells. DPPA-2 has binding sites for both POU and Sox-2 protein domains and is expressed primarily in the developing germ line following gastrulation. These associations suggest that this protein participates in a very strong supportive role in maintaining cell pluripotentiality. In addition to its role as a pluripotency marker, more recent research using cancer cells suggests that DPPA-2 may also act as an embryo-cancer antigen—pointing to the presence of abnormal cell growth and the formation of cancerous cells.
Specificity
Reactivity with other species has not been tested.
This monoclonal antibody recognizes the mouse DPPA-2 protein.
Immunogen
Full length recombinant mouse DPPA-2 protein.
Application
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Pluripotent & Early Differentiation
This Anti-DPPA-2 Antibody, clone 6C1.2 is validated for use in WB for the detection of DPPA-2.
Quality
(Based upon representative data from a previous lot).
Western Blotting: Recommended working concentration is 2 µg/mL.
Western Blotting: Recommended working concentration is 2 µg/mL.
Target description
~34 kDa
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4) and
150 mM NaCl with 0.05% sodium azide.
150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Mélanie Eckersley-Maslin et al.
Genes & development, 33(3-4), 194-208 (2019-01-30)
The molecular regulation of zygotic genome activation (ZGA) in mammals remains an exciting area of research. Primed mouse embryonic stem cells contain a rare subset of "2C-like" cells that are epigenetically and transcriptionally similar to the two-cell embryo and thus
Oana Kubinyecz et al.
Development (Cambridge, England), 148(24) (2021-12-22)
Zygotic genome activation (ZGA) represents the initiation of transcription following fertilisation. Despite its importance, we know little of the molecular events that initiate mammalian ZGA in vivo. Recent in vitro studies in mouse embryonic stem cells have revealed developmental pluripotency
Celia Alda-Catalinas et al.
Cell systems, 11(1), 25-41 (2020-07-08)
Zygotic genome activation (ZGA) is an essential transcriptional event in embryonic development that coincides with extensive epigenetic reprogramming. Complex manipulation techniques and maternal stores of proteins preclude large-scale functional screens for ZGA regulators within early embryos. Here, we combined pooled
Ilan Theurillat et al.
Cell reports, 32(11), 108146-108146 (2020-09-17)
Post-translational modification by SUMO is a key regulator of cell identity. In mouse embryonic fibroblasts (MEFs), SUMO impedes reprogramming to pluripotency, while in embryonic stem cells (ESCs), it represses the emergence of totipotent-like cells, suggesting that SUMO targets distinct substrates
Charles Hernandez et al.
Cell stem cell, 23(3), 396-411 (2018-08-28)
As somatic cells are converted into induced pluripotent stem cells (iPSCs), their chromatin is remodeled to a pluripotent configuration with unique euchromatin-to-heterochromatin ratios, DNA methylation patterns, and enhancer and promoter status. The molecular machinery underlying this process is largely unknown.
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