345797

α1-3,6-Galactosidase, Recombinant, E. coli

Catalyzes the hydrolysis of α1,3-linked and α1,6-linked, non-reducing terminal galactose from complex carbohydrates and glycoproteins.

• Synonym(s): α1-3,6-Galactosidase, Recombinant, E. coli, α1-3,6-D-Galactoside Galactohydrolase
• CAS Number: 9025-35-8
• Enzyme Commission number: 3.2.1.22 (BRENDA, IUBMB)
• MDL number: MFCD00130486
• UNSPSC Code: 12352202
• NACRES: NA.54

Properties

• recombinant: expressed in E. coli
• Quality Level: 100
• form: liquid
• specific activity: ≥30 units/mg protein; ≥85 units/mL
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• foreign activity: Proteases, none detected
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Recombinant α1-3,6-galactosidase expressed in E. coli. Catalyzes the hydrolysis of α1,3-linked and α1,6-linked, non-reducing terminal galactose from complex carbohydrates and glycoproteins. Particularly efficient in removing α-linked galactose under conditions where the pH must be neutral or above, as with live cells.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-α-D-galactopyranoside per min at 25°C, pH 6.5.

Physical form

In 50 mM sodium phosphate buffer, pH 7.5.

Other Notes

Prime, S., et al. 1996. J. Chromatogr. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable