Recommended Products
manufacturer/tradename
Chemicon®
technique(s)
protein extraction: suitable
application(s)
sample preparation
shipped in
dry ice
storage temp.
2-8°C
Application
CHEMICON′s Whole Cell Extraction Kit (Catalog No. 2910) provides a simple and convenient method for the preparation of whole cell extractions for use in experimental studies of various cellular proteins.
This kit is recommended for use with CHEMICON′s Rac Activation Assay Kit (Catalog No. SGT425), Cdc42 Activation Assay Kit (Catalog No. SGT430), Ras Activation Assay Kit (Catalog No. SGT435), and CHEMICON′s Transcription Factor Assays when purified nuclear extracts are not required.
For Research Use Only; Not for use in diagnostic procedures
This kit is recommended for use with CHEMICON′s Rac Activation Assay Kit (Catalog No. SGT425), Cdc42 Activation Assay Kit (Catalog No. SGT430), Ras Activation Assay Kit (Catalog No. SGT435), and CHEMICON′s Transcription Factor Assays when purified nuclear extracts are not required.
For Research Use Only; Not for use in diagnostic procedures
Research Category
All
All
Components
Whole Cell Extraction Buffer, 5x: - (Part No. 90493) Two vials containing 10 mL of 5x concentrated buffer. Dilute to 1x final concentration with ddH2O prior to use.
Protease Inhibitor Cocktail: - (Part No. 90492) One vial containing 100μL of protease inhibitors in DMSO for use with mammalian cells and tissue extracts. A mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, and aspartic acid proteases and aminopeptidases. Contains 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), pepstatin A, E-64, bestatin, leupeptin, and aprotinin. Contains no metal chelators. Prior to use, dilute 1/1000 in 1x Whole Cell Extraction Buffer.
Protease Inhibitor Cocktail: - (Part No. 90492) One vial containing 100μL of protease inhibitors in DMSO for use with mammalian cells and tissue extracts. A mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, and aspartic acid proteases and aminopeptidases. Contains 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), pepstatin A, E-64, bestatin, leupeptin, and aprotinin. Contains no metal chelators. Prior to use, dilute 1/1000 in 1x Whole Cell Extraction Buffer.
Storage and Stability
· The 5x Whole Cell Extraction Buffer can be stored at 2-8°C until expiration date.
· The prepared 1x Whole Cell Extraction Buffer (with optional additives) should be stored at -20°C for up to one month.
· The undiluted Protease Inhibitor Cocktail can be stored at -20°C until the expiration date.
· The prepared 1x Whole Cell Extraction Buffer (with optional additives) should be stored at -20°C for up to one month.
· The undiluted Protease Inhibitor Cocktail can be stored at -20°C until the expiration date.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Yuxuan Xiao et al.
Biochemical and biophysical research communications, 478(2), 919-923 (2016-08-16)
Sumoylation (a covalent modification by Small Ubiquitin-like Modifiers or SUMO proteins) has been implicated in the regulation of various cellular events including cell cycle progression. We have recently identified CDK1, a master regulator of mitosis and meiosis, as a SUMO
Yuxuan Xiao et al.
Reproduction (Cambridge, England), 151(2), 149-166 (2015-12-25)
Recent findings suggest diverse and potentially multiple roles of small ubiquitin-like modifier (SUMO) in testicular function and spermatogenesis. However, SUMO targets remain uncharacterized in the testis due to the complex multicellular nature of testicular tissue, the inability to maintain and
Andrew J Schneider et al.
Toxicological sciences : an official journal of the Society of Toxicology, 141(1), 176-187 (2014-06-15)
In utero exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) causes ventral prostate agenesis in C57BL/6J mice by preventing ventral prostatic budding in the embryonic urogenital sinus (UGS). TCDD (5 μg/kg, po) administered to pregnant dams on embryonic day 15.5 (E15.5) activates the aryl
Controlled release hydrophilic matrix tablet formulations of isoniazid: design and in vitro studies.
Praveen S Hiremath et al.
AAPS PharmSciTech, 9(4), 1171-1178 (2008-11-19)
The aim of the present investigation was to develop oral controlled release matrix tablet formulations of isoniazid using hydroxypropyl methylcellulose (HPMC) as a hydrophilic release retardant polymer and to study the influence of various formulation factors like proportion of the
Yuxuan Xiao et al.
Biochemical and biophysical research communications, 487(3), 640-645 (2017-04-25)
The meiotic G2/M1 transition is mostly regulated by posttranslational modifications, however, the cross-talk between different posttranslational modifications is not well-understood, especially in spermatocytes. Sumoylation has emerged as a critical regulatory event in several developmental processes, including reproduction. In mouse oocytes
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