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Millipore

ReadyPlate™ Listeria Agar

Ready-to-use 90mm plates for the detection of Listeria monocytogenes and other Listeria sp. from food and environmental samples acc to ISO 11290

Synonym(s):

Listeria Agar according to Ottaviani and Agosti

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About This Item

UNSPSC Code:
41106217
NACRES:
NA.74

Agency

ISO 11290

Quality Level

300

form

plate (ready-to-use)

feature

ready-to-use

manufacturer/tradename

ReadyPlate™

packaging

pkg of (90 mm plate (20 plates per box))

pH

7.2 ( in H2O)

application(s)

environmental
food and beverages
microbiology
water monitoring

storage temp.

2-8°C

suitability

Listeria spp.

General description

ReadyPlate™ Listeria Agar plates are ready-to-use, sterile 90mm plates for the enumeration, detection, and identification of Listeria monocytogenes and other Listeria sp. from food and feeding stuff. This medium contains the chromogenic compound 5-bromo-4- chloro-3-indolyl-β-D glucopyranoside, a substrate for the detection of β-glucosidase. This enzyme is commonly produced by all Listeria sp. that appear on the medium as blue-green colored colonies. Differentiation of Listeria monocytogenes from other Listeria sp. is achieved through the production of a phosphatidylinositol-specific phospholipase C (PI-PLC). Listeria monocytogenes hydrolyses the specific purified substrate added to the medium producing an opaque halo around the colonies.
This basic pH of the medium, ensures good growth for a broad range of bacteria. The addition of inhibitors results in the reduction of the growth of the majority of concomitant Gram-positive and Gram-negative pathogens, as well as of yeasts and fungi. Selectivity is obtained by the addition of lithium chloride, nalidixic acid, and cycloheximide.

Application

Listeria Agar are ready-to-use, sterile 90mm plates for isolation and differentiation of Listeria monocytogenes and other Listeria sp. from food and animal feed, environmental samples in the area of food production, food handlings and other materials.

Legal Information

READYPLATE is a trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 2

Certificates of Analysis (COA)

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Articles

Detection of Listeria monocytogenes in Food and Environmental Samples

Detection of Listeria monocytogenes in food and environmental samples according to ISO 11290-1, FDA-BAM and USDA-FSIS

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