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Sigma-Aldrich

Azide agarose

Synonym(s):

Azide functionalized agarose

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About This Item

UNSPSC Code:
12352125
NACRES:
NA.22

form

liquid

Quality Level

100

reaction suitability

reaction type: click chemistry

refractive index

n/D 1.343

density

0.978 g/mL

storage temp.

2-8°C

Application

Azide agarose is a 6% crosslinked agarose resin that is activated with azide functional groups for covalent capture of alkyne-tagged biomolecules via copper(I)-catalyzed alkyne-azide or copper(I)-free strain-promoted alkyne-azide click chemistry approach. This product can be used for either a batch protocol or a low-pressure column method.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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?Clickable? agarose for affinity chromatography.
Punna S, et al.
Bioconjugate Chemistry, 16(6), 1536-1541 (2005)
Sreenivas Punna et al.
Bioconjugate chemistry, 16(6), 1536-1541 (2005-11-17)
Successful purification of biological molecules by affinity chromatography requires the attachment of desired ligands to biocompatible chromatographic supports. The Cu(I)-catalyzed cycloaddition of azides and alkynes-the premier example of "click chemistry"-is an efficient way to make covalent connections among diverse molecules

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