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Custom qPCR Probes

Design and Order DNA, Locked Nucleic Acid, and BHQ Probes for qPCR

Synonym(s):

BHQ, BHQ Probes, Black Hole Quencher, Locked Nucleic Acid probes, Probes, Real-Time PCR Probes, locked nucleic acid, qPCR assays, qPCR designs

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UNSPSC Code:
41105904
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Custom Oligos & qPCR Probes Online Tools
Product Resources Title
Custom DNA and RNA Oligos & qPCR Probes Product Resources

Dual-Labeled Probes

  • Amounts: 1, 3, 5, and 10 OD
  • Purification: HPLC
  • Sequence Lengths: 15 - 40 bases
  • Modifications: Locked Nucleic Acid available
  • Dyes: Comprehensive offering of reporters and quenchers
  • Quality Control: 100% mass spectrometry
  • Format: Supplied dry in amber tubes
  • Learn more
Order

Molecular Beacons

  • Amounts: 1, 3, 5, and 10 OD
  • Purification: HPLC
  • Sequence Lengths: 15 - 40 bases
  • Modifications: Locked Nucleic Acid available
  • Dyes: Comprehensive offering of reporters and quenchers
  • Quality Control: 100% mass spectrometry
  • Format: Supplied dry in amber tubes
  • Learn more
Order

LightCycler® Probes

  • Amounts: 0.1, 0.25, 1.5, and 15 OD
  • Purification: HPLC
  • Sequence Lengths: 15 - 40 bases
  • Modifications: Locked Nucleic Acid available
  • Dyes: Probe 1 fluorescein (3′ end); Probe 2 phosphate (3′ end) and LC Red 610, 640, 670 or 705 (5′ end)
  • Quality Control: 100% mass spectrometry
  • Format: Supplied dry in amber tubes
  • Learn more
Order

Scorpions® Probes

  • Amounts: 1, 5, and 5 OD
  • Purification: HPLC
  • Sequence Lengths: 30 - 60 bases
  • Modifications: Locked Nucleic Acid available
  • Dyes: Comprehensive offering of reporters and quenchers
  • Quality Control: 100% mass spectrometry
  • Format: Supplied dry in amber tubes
  • Learn more
Order

Locked Nucleic Acid

  • Available for all Custom qPCR Probe Types
  • Increased thermal stability and hybridization specificity
  • Easier and more specific designs for problematic target sequences
  • Learn more
Order

Black Hole Quencher™ (BHQ™)

  • Available for all Custom qPCR Probe types
  • No native fluorescence (emits heat instead of light), resulting in lower background fluorescence
  • Increased signal-to-noise ratio, providing higher sensitivity
  • Enables a wider choice of reporters for multiplexing
  • Learn more
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Ana F Almeida-Santos et al.
Scientific reports, 9(1), 473-473 (2019-01-27)
The absence of companion may jeopardize mental health in social animals. Here, we tested the hypothesis that social isolation impairs social recognition memory by altering the excitability and the dialog between the olfactory bulb (OB) and the dorsal hippocampus (dHIP).
Rajeev Gupta et al.
Cell stem cell, 26(4), 527-541 (2020-03-21)
Umbilical cord blood (UCB) has had considerable impact in pediatric stem cell transplantation, but its wider use is limited in part by unit size. Long-term ex vivo culture offers one approach to increase engraftment capacity by seeking to expand stem and

Articles

Dual-Labeled Probes in Digital PCR

Learn more about how digital PCR (dPCR) is used for absolute quantification and for analysis of minority sequences.

Oligonucleotide Purification

Learn about the importance of oligonucleotide purification, recommended methods, common techniques and applications, and key steps in the processes.

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LightCycler® Probes

LightCycler probes are highly sensitive and sequence-specific fluorescent probes designed for use with the Roche LightCycler instruments.

No Template Control Oligos (NTCO)

If laboratory handling error has been ruled out, contaminated primers and probes may be to blame for a signal from the qPCR no template control.

OligoArchitect™ Consultative

For more complex and demanding applications, utilize our consultative service to ensure the success of your assay.

Oligonucleotide QA: Quality Management System

QMS features certificates, document control, vendor management, and corrective actions, ensuring quality management standards.

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