58297
SUPELCOSIL™ LC-8 (5 µm) HPLC Columns
L × I.D. 25 cm × 4.6 mm, HPLC Column
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product name
SUPELCOSIL™ LC-8 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
Agency
suitable for USP L7
feature
endcapped
manufacturer/tradename
SUPELCOSIL™
extent of labeling
6.0% carbon loading
parameter
≤70 °C temp. range
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
L × I.D.
25 cm × 4.6 mm
surface area
170 m2/g
surface coverage
surface coverage 3.2 μmol/m2
matrix
silica gel, spherical particle platform
matrix active group
C8 (octyl) phase
particle size
5 μm
pore size
120 Å
pH range
2-7.5
application(s)
food and beverages
separation technique
reversed phase
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General description
A phase less hydrophobic than C18. Provides less retention of both polar and non-polar compounds than C18. Use a mobile phase containing 5% less organic modifier for the C8 column than C18. Polar compounds are, relatively, more strongly retained on C8 than C18 columns.
Legal Information
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
Application
guard cartridge
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A HPLC-UV determination of clobazam and N-desmethylclobazam in human serum and urine is presented. After simple liquid-liquid extraction with dichloromethane the compounds and an internal standard diazepam were separated on a Supelcosil LC-8-DB column at ambient temperature under isocratic conditions
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application for air monitoring, application for HPLCOur team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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