SAB4200537

Anti-Claudin-5 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-AWAL, Anti-BEC1, Anti-CLDN5, Anti-CPETRL1, Anti-TMVCF
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~23 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): immunohistochemistry: 20 μg/mL using formalin-fixed, paraffin-embedded human heart; western blot: 1-2 μg/mL using extracts of OVCAR-3 cells
• UniProt accession no.: O00501
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CLDN5(7122)

Description

General description

Claudin-5 is a member of claudin family. It is also known as CLDN5, beclin 1, TMVCF and CPETRL1 and is highly expressed in endothelial cells (ECs), including the blood-brain barrier.

Claudin-5 is encoded by the gene mapped to human chromosome 22q11.21.

Specificity

Anti-Claudin-5specifically recognizes human Claudin-5.

Immunogen

synthetic peptide corresponding to an internal sequence of human claudin-5, conjugated to KLH. The corresponding sequence is highly conserved (single amino acid substitution) in mouse and rat claudin-5.

Application

Anti-Claudin-5 antibody produced in rabbit has been used in immunofluorescence staining.

Anti-Claudin-5 antibody produced in rabbit may be used in several immunochemical techniques including immunoblotting (~23 kDa) and immunohistochemistry.

Biochem/physiol Actions

Claudin-5 gene is a downstream target of ETS-related gene (ERG), a transcription factor that regulates endothelial cell-restricted genes and is markedly repressed in response to inflammatory stimuli. Knockout of claudin-5 in mice is lethal, resulting in disruption of in the blood-brain barrier. Claudin-5 has been shown to be overexpressed in various tumors including lung adenocarcinomas but undetectable in squamous cells cell carcinomas.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable