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Sucrose Phosphorylase

Name: Sucrose Phosphorylase
Brand: SIGMA

recombinant, expressed in E. coli, lyophilized powder, ≥45 units/mg solid

Synonym(s):

SPase, disaccharide glucosyltransferase, sucrose glucosyltransferase, Sucrose:orthophosphate α-D-glucosytransferase

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About This Item

CAS Number:

9074-06-0

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

2.4.1.7 (BRENDA, IUBMB)

MDL number:

MFCD00132397

Specific activity:

≥45 units/mg solid

Recombinant:

expressed in E. coli

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Properties

recombinant

expressed in E. coli

Quality Segment

200

form

lyophilized powder

specific activity

≥45 units/mg solid

mol wt

56 kDa by SDS-PAGE

shipped in

wet ice

storage temp.

−20°C

Description

General description

Research area: Cell signaling
Sucrose Phosphorylase belongs to glycoside hydrolase, GH13 family. It comprises of four domains with the glucose anomeric carbon-binding site and a glucoside-binding site. The active site residues include Asp192 and Glu232. It is majorly produced by bifidobacteria and lactic acid bacteria. The cross-linked sucrose phosphorylase aggregates is thermostable and could be exploited for industrial catalysis of glycosylation.[1]

Application

Sucrose Phosphorylase has been used in sucrose determination in wheat plant and in sucrose hydrogen production.

Sucrose phosphorylase has been used:

To assess the enzymatic synthesis of stable, odorless, and powdered furanone glucosides. [2]
To investigate the novel transglucosylating reaction with carboxylic compounds. [3]
In sucrose determination in wheat plant and in sucrose hydrogen production.

Biochem/physiol Actions

Sucrose phosphorylase catalyzes the reversible conversion of sucrose (α-D-glucopyranosyl-1,2-β-D-fructofuranoside) and phosphate into D-fructose and α-D-glucose 1-phosphate. [4]This reaction plays a crucial role in generating the vital glucose component through sucrose metabolism.(1)

Physical form

Contains sucrose as stabilizer.

Other Notes

One unit will produce 1.0 μmole of D-fructose from sucrose per min with the corresponding reduction of NADP to NADPH at pH 7.6, at 25 °C.

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This Item	M8284	A9934	P6635
Sigma-Aldrich S0937 Sucrose Phosphorylase	Sigma-Aldrich M8284 Maltose Phosphorylase from Enterococcus sp.	Sigma-Aldrich A9934 Aminopeptidase I from Streptomyces griseus	Sigma-Aldrich P6635 Phosphorylase b from rabbit muscle
specific activity ≥45 units/mg solid	specific activity ≥9 units/mg solid	specific activity ≥200 units/mg protein	specific activity ≥20 units/mg protein
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant -	recombinant -
mol wt 56 kDa by SDS-PAGE	mol wt 90 kDa by SDS-PAGE	mol wt 21 kDa by gel filtration, 33 kDa by SDS-PAGE	mol wt 97,200 Da by calculation
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
shipped in wet ice	shipped in -	shipped in -	shipped in -

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

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