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RAB0631

Sigma-Aldrich

Human C5a / Complement C5a ELISA Kit

for serum, plasma, and cell culture supernatants

Synonym(s):

Complement C5a

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 31 pg/mL
standard curve range: 31.25-2000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... C5(727)

General description

Human complement 5a (C5a) is generated during complement activation from the α-chain of complement C5. It binds to a signaling G-protein coupled receptor (GPCR), inducing neutrophil chemotaxis and endothelial cell activation. C5a also triggers an oxidative burst in macrophages and neutrophils and induces release of histamine in basophils and mast cells.
The antibody pair provided in this kit recognizes human Complement Component C5a.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Michael T Eadon et al.
Immunology, 143(3), 363-373 (2014-05-08)
Blood-brain barrier (BBB) disturbance is a crucial occurrence in many neurological diseases, including systemic lupus erythematosus (SLE). Our previous studies showed that experimental lupus serum altered the integrity of the mouse brain endothelial layer, an important constituent of the BBB.
Molecular and expression analysis of complement component C5 in the nurse shark (Ginglymostoma cirratum) and its predicted functional role.
Matthew G, et al.
Fish & Shellfish Immunology, 27(1), 40-49 (2009)
New developments in C5a receptor signaling
Sarma J V and Peter A W
Cell health and cytoskeleton, 4, 73-82 (2012)
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