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P6649

Millipore

Protein A–Sepharose 6MB

aqueous ethanol suspension

Synonym(s):

Protein A–Agarose macrobeads

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About This Item

MDL number:
UNSPSC Code:
41106500
NACRES:
NA.56

form

aqueous ethanol suspension

extent of labeling

~1 mg per mL

matrix activation

cyanogen bromide

matrix attachment

amino

matrix spacer

1 atom

capacity

~6 mg/mL binding capacity (human IgG)

storage temp.

2-8°C

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General description

P6649-10ml′s updated product number is GE17-0469-01

Application

Protein A-Sepharose is used for affinity chromatography, antibody purification and characterization, immunoaffinity matrices, protein chromatography, protein A, G and L resins, and recombinant protein expression and analysis. Protein A-Sepharose has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.
Protein A–Sepharose 6MB has been used in immunoprecipitation.

Physical form

Suspension in 20% ethanol

Legal Information

Sepharose is a trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

100.4 °F

Flash Point(C)

38 °C


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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G V Hillyer et al.
The American journal of tropical medicine and hygiene, 38(3), 547-552 (1988-05-01)
Total RNA containing messenger RNA has been isolated from adult Fasciola hepatica and translated in vitro using the rabbit reticulocyte lysate system. L-[35S]-methionine labeled translation products have been immunoprecipitated with sera collected from rabbits infected with F. hepatica, rabbits immunized
Nikolai Peschek et al.
The EMBO journal, 38(16), e101650-e101650 (2019-07-18)
Small regulatory RNAs (sRNAs) are crucial components of many stress response systems. The envelope stress response (ESR) of Gram-negative bacteria is a paradigm for sRNA-mediated stress management and involves, among other factors, the alternative sigma factor E (σE ) and
Gaurav Dugar et al.
Molecular cell, 69(5), 893-905 (2018-03-03)
Cas9 nucleases naturally utilize CRISPR RNAs (crRNAs) to silence foreign double-stranded DNA. While recent work has shown that some Cas9 nucleases can also target RNA, RNA recognition has required nuclease modifications or accessory factors. Here, we show that the Campylobacter
Verena Pfeiffer et al.
Molecular microbiology, 66(5), 1174-1191 (2007-11-01)
The Salmonella pathogenicity island (SPI-1) encodes approximately 35 proteins involved in assembly of a type III secretion system (T3SS) which endows Salmonella with the ability to invade eukaryotic cells. We have discovered a novel SPI-1 gene, invR, which expresses an
Aroa Rey Campa et al.
Nucleic acids research, 49(16), 9508-9525 (2021-08-18)
CRISPR-Cas systems provide bacteria with adaptive immunity against phages and plasmids; however, pathways regulating their activity are not well defined. We recently developed a high-throughput genome-wide method (SorTn-seq) and used this to uncover CRISPR-Cas regulators. Here, we demonstrate that the

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